KHARON is an essential cytoskeletal protein involved in the trafficking of flagellar membrane proteins and cell division in african trypanosomes

Marco A. Sanchez, Khoa D. Tran, Jessica Valli, Sam Hobbs, Errin Johnson, Eva Gluenz, Scott M. Landfear*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

African trypanosomes and related kinetoplastid parasites selectively traffic specific membrane proteins to the flagellar membrane, but the mechanisms for this trafficking are poorly understood. We show here that KHARON, a protein originally identified in Leishmania parasites, interacts with a putative trypanosome calcium channel and is required for its targeting to the flagellar membrane. KHARON is located at the base of the flagellar axoneme, where it likely mediates targeting of flagellar membrane proteins, but is also on the subpellicular microtubules and the mitotic spindle. Hence, KHARON is probably a multifunctional protein that associates with several components of the trypanosome cytoskeleton. RNA interference-mediated knockdown of KHARON mRNA results in failure of the calcium channel to enter the flagellar membrane, detachment of the flagellum from the cell body, and disruption of mitotic spindles. Furthermore, knockdown of KHARON mRNA induces a lethal failure of cytokinesis in both bloodstream (mammalian host) and procyclic (insect vector) life cycle stages, and KHARON is thus critical for parasite viability.
Original languageEnglish
Pages (from-to)19760-19773
Number of pages14
JournalJournal of Biological Chemistry
Volume291
Issue number38
DOIs
Publication statusPublished - 16 Sept 2016

Keywords

  • calcium channel
  • cytoskeleton
  • membrane protein
  • mitotic spindle
  • trafficking
  • Trypanosoma brucei

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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