Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

Rory Duncan, David K. Apps, Michele P. Learmonth, Michael J. Shipston, Robert H. Chow

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalBiochemical Journal
Volume351
Issue number1
DOIs
Publication statusPublished - 1 Oct 2000

Keywords

  • Adrenal Medulla
  • Animals
  • Antibodies, Monoclonal
  • Antigens
  • Brain
  • Calcium-Binding Proteins
  • Cattle
  • Chromaffin Cells
  • Cross Reactions
  • Immune Sera
  • Mice
  • Mitochondria
  • Molecular Weight
  • Nerve Tissue Proteins
  • Protein Biosynthesis
  • RNA, Messenger
  • Reverse Transcriptase Polymerase Chain Reaction

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