Is double C2 protein (DOC2) expressed in bovine adrenal medulla?: A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

Rory Duncan; David K. Apps; Michele P. Learmonth; Michael J. Shipston; Robert H. Chow

doi:10.1042/bj3510033

Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

Rory Duncan, David K. Apps, Michele P. Learmonth, Michael J. Shipston, Robert H. Chow

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.

Original language	English
Pages (from-to)	33-37
Number of pages	5
Journal	Biochemical Journal
Volume	351
Issue number	1
DOIs	https://doi.org/10.1042/bj3510033
Publication status	Published - 1 Oct 2000

Keywords

Adrenal Medulla
Animals
Antibodies, Monoclonal
Antigens
Brain
Calcium-Binding Proteins
Cattle
Chromaffin Cells
Cross Reactions
Immune Sera
Mice
Mitochondria
Molecular Weight
Nerve Tissue Proteins
Protein Biosynthesis
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction

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10.1042/bj3510033

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title = "Is double C2 protein (DOC2) expressed in bovine adrenal medulla?: A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen",

abstract = "We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.",

keywords = "Adrenal Medulla, Animals, Antibodies, Monoclonal, Antigens, Brain, Calcium-Binding Proteins, Cattle, Chromaffin Cells, Cross Reactions, Immune Sera, Mice, Mitochondria, Molecular Weight, Nerve Tissue Proteins, Protein Biosynthesis, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction",

author = "Rory Duncan and Apps, {David K.} and Learmonth, {Michele P.} and Shipston, {Michael J.} and Chow, {Robert H.}",

year = "2000",

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doi = "10.1042/bj3510033",

language = "English",

volume = "351",

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journal = "Biochemical Journal",

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publisher = "Portland Press Ltd.",

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T1 - Is double C2 protein (DOC2) expressed in bovine adrenal medulla?

T2 - A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

AU - Duncan, Rory

AU - Apps, David K.

AU - Learmonth, Michele P.

AU - Shipston, Michael J.

AU - Chow, Robert H.

PY - 2000/10/1

Y1 - 2000/10/1

N2 - We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.

AB - We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.

KW - Adrenal Medulla

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigens

KW - Brain

KW - Calcium-Binding Proteins

KW - Cattle

KW - Chromaffin Cells

KW - Cross Reactions

KW - Immune Sera

KW - Mice

KW - Mitochondria

KW - Molecular Weight

KW - Nerve Tissue Proteins

KW - Protein Biosynthesis

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1042/bj3510033

DO - 10.1042/bj3510033

M3 - Article

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SN - 0264-6021

VL - 351

SP - 33

EP - 37

JO - Biochemical Journal

JF - Biochemical Journal

IS - 1

ER -

Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

Abstract

Keywords

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