TY - JOUR
T1 - Investigation of albumin properties in patients with chronic renal failure
AU - Pieniazek, Anna
AU - Brzeszczywska, Joanna
AU - Kruszynska, Ilona
AU - Gwozdzinski, Krzysztof
PY - 2009
Y1 - 2009
N2 - The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process. © 2009 Informa UK Ltd All rights reserved.
AB - The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process. © 2009 Informa UK Ltd All rights reserved.
KW - Albumin
KW - Chronic renal failure
KW - Glycosylation
KW - Haemodialysis
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=70450247117&partnerID=8YFLogxK
U2 - 10.1080/10715760903165003
DO - 10.1080/10715760903165003
M3 - Article
C2 - 19707919
SN - 1071-5762
VL - 43
SP - 1008
EP - 1018
JO - Free Radical Research
JF - Free Radical Research
IS - 10
ER -