Integration and amplification of a cyclodextrin glycosyltransferase gene from Thermoanaerobacter sp. ATCC 53627 on the Bacillus subtilis chromosome

Steen T. Jørgensen, Martin Tangney, P. L. Jørgensen, Børge Diderichsen

    Research output: Contribution to journalArticle

    Abstract

    A plasmid was constructed containing the replication functions of pUC19, and the cgtA gene from Thermoanaerobacter sp. ATCC 53627, flanked by the dal gene from Bacillus subtilis and a sequence downstream from this gene. This was transformed into a Dal- B. subtilis strain, selecting for Dal+ transformants, which contained the cgtA gene in single copy integrated in the B. subtilis chromosome. The gene was subsequently amplified by a method which ensured that there was no functional plasmid replication system on the integrated DNA. The amplified structure was stable in the absence of selection pressure.

    Original languageEnglish
    Pages (from-to)15-19
    Number of pages5
    JournalBiotechnology Techniques
    Volume12
    Issue number1
    DOIs
    Publication statusPublished - 1998

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