Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

A. L. Bernassau; M. Al-Rawhani; J. Beeley; D. R. S. Cumming

doi:10.1063/1.4846395

Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

A. L. Bernassau^*
, M. Al-Rawhani
, J. Beeley
, D. R. S. Cumming

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

112 Downloads (Pure)

Abstract

A compact hybrid system has been developed to position and detect fluorescent micro-particles by combining a Single Photon Avalanche Diode (SPAD) imager with an acoustic manipulator. The detector comprises a SPAD array, light-emitting diode (LED), lenses, and optical filters. The acoustic device is formed of multiple transducers surrounding an octagonal cavity. By stimulating pairs of transducers simultaneously, an acoustic landscape is created causing fluorescent microparticles to agglomerate into lines. The fluorescent pattern is excited by a low power LED and detected by the SPAD imager. Our technique combines particle manipulation and visualization in a compact, low power, portable setup.

Original language	English
Article number	244103
Number of pages	4
Journal	Applied Physics Letters
Volume	103
Issue number	24
DOIs	https://doi.org/10.1063/1.4846395
Publication status	Published - 9 Dec 2013

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Copyright (2013) American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Appl. Phys. Lett. 103, 244103 (2013) and may be found at http://dx.doi.org/10.1063/1.4846395
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Cite this

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Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

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