Integrated ultrasonic particle positioning and low excitation light fluorescence imaging

A. L. Bernassau, M. Al-Rawhani, J. Beeley, D. R. S. Cumming

Research output: Contribution to journalArticle

42 Downloads (Pure)

Abstract

A compact hybrid system has been developed to position and detect fluorescent micro-particles by combining a Single Photon Avalanche Diode (SPAD) imager with an acoustic manipulator. The detector comprises a SPAD array, light-emitting diode (LED), lenses, and optical filters. The acoustic device is formed of multiple transducers surrounding an octagonal cavity. By stimulating pairs of transducers simultaneously, an acoustic landscape is created causing fluorescent microparticles to agglomerate into lines. The fluorescent pattern is excited by a low power LED and detected by the SPAD imager. Our technique combines particle manipulation and visualization in a compact, low power, portable setup.

Original languageEnglish
Article number244103
Number of pages4
JournalApplied Physics Letters
Volume103
Issue number24
DOIs
Publication statusPublished - 9 Dec 2013

Cite this