Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

O. Sparagano; G. R. Loria; M. J. Gubbels; A. P. De Vos; S. Caracappa; F. Jongejan

Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

O. Sparagano
, G. R. Loria
, M. J. Gubbels
, A. P. De Vos
, S. Caracappa
, F. Jongejan

Research output: Contribution to journal › Article › peer-review

Abstract

A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

Original language	English
Pages (from-to)	533-539
Number of pages	7
Journal	Annals of the New York Academy of Sciences
Volume	916
Publication status	Published - 2000

Cite this

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title = "Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy",

abstract = "A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8\% and 23.5\% in April and November, respectively.",

author = "O. Sparagano and Loria, \{G. R.\} and Gubbels, \{M. J.\} and \{De Vos\}, \{A. P.\} and S. Caracappa and F. Jongejan",

year = "2000",

language = "English",

volume = "916",

pages = "533--539",

journal = "Annals of the New York Academy of Sciences",

issn = "0077-8923",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

AU - Sparagano, O.

AU - Loria, G. R.

AU - Gubbels, M. J.

AU - De Vos, A. P.

AU - Caracappa, S.

AU - Jongejan, F.

PY - 2000

Y1 - 2000

N2 - A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

AB - A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

M3 - Article

SN - 0077-8923

VL - 916

SP - 533

EP - 539

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

Abstract

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