Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

O. Sparagano, G. R. Loria, M. J. Gubbels, A. P. De Vos, S. Caracappa, F. Jongejan

    Research output: Contribution to journalArticle

    Abstract

    A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

    Original languageEnglish
    Pages (from-to)533-539
    Number of pages7
    JournalAnnals of the New York Academy of Sciences
    Volume916
    Publication statusPublished - 2000

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    Sparagano, O., Loria, G. R., Gubbels, M. J., De Vos, A. P., Caracappa, S., & Jongejan, F. (2000). Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy. Annals of the New York Academy of Sciences, 916, 533-539.