Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

O. Sparagano, G. R. Loria, M. J. Gubbels, A. P. De Vos, S. Caracappa, F. Jongejan

    Research output: Contribution to journalArticle

    Abstract

    A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

    Original languageEnglish
    Pages (from-to)533-539
    Number of pages7
    JournalAnnals of the New York Academy of Sciences
    Volume916
    Publication statusPublished - 2000

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    Theileria
    Babesia
    Theileria buffeli
    Theileria mutans
    Anaplasma centrale
    Anaplasma ovis
    Italy
    Babesia divergens
    Ehrlichia ruminantium
    Theileria parva
    Theileria annulata
    Babesia bigemina
    Babesia bovis
    Trypanosoma brucei
    Anaplasma marginale
    cattle
    blood
    Sicily
    cross reaction
    hybridization

    Cite this

    Sparagano, O., Loria, G. R., Gubbels, M. J., De Vos, A. P., Caracappa, S., & Jongejan, F. (2000). Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy. Annals of the New York Academy of Sciences, 916, 533-539.
    Sparagano, O. ; Loria, G. R. ; Gubbels, M. J. ; De Vos, A. P. ; Caracappa, S. ; Jongejan, F. / Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy. In: Annals of the New York Academy of Sciences. 2000 ; Vol. 916. pp. 533-539.
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    abstract = "A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8{\%} and 23.5{\%} in April and November, respectively.",
    author = "O. Sparagano and Loria, {G. R.} and Gubbels, {M. J.} and {De Vos}, {A. P.} and S. Caracappa and F. Jongejan",
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    Sparagano, O, Loria, GR, Gubbels, MJ, De Vos, AP, Caracappa, S & Jongejan, F 2000, 'Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy', Annals of the New York Academy of Sciences, vol. 916, pp. 533-539.

    Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy. / Sparagano, O.; Loria, G. R.; Gubbels, M. J.; De Vos, A. P.; Caracappa, S.; Jongejan, F.

    In: Annals of the New York Academy of Sciences, Vol. 916, 2000, p. 533-539.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy

    AU - Sparagano, O.

    AU - Loria, G. R.

    AU - Gubbels, M. J.

    AU - De Vos, A. P.

    AU - Caracappa, S.

    AU - Jongejan, F.

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    N2 - A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

    AB - A reverse line blot hybridization (RLB) test was developed to specifically identify six Theileria spp. (T. annulata, T. parva, T. mutans, T. velifera, T. taurotragi, and T. buffeli/orientalis) and three Babesia spp. (B. bovis, B. bigemina, and B. divergens). No cross reaction was observed with other livestock pathogens (such as Anaplasma marginale, A. centrale, A. ovis, Cowdria ruminantium, Trypanosoma brucei, T. congolense, and T. vivax). This method was used to test bovine blood samples collected in Sicily in April and November, 1998. Preliminary results indicated that T. annulata and T. buffeli/orientalis were the main species observed in cattle blood. Babesia species represented 1.8% and 23.5% in April and November, respectively.

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    Sparagano O, Loria GR, Gubbels MJ, De Vos AP, Caracappa S, Jongejan F. Integrated molecular diagnosis of Theileria and Babesia species of cattle in Italy. Annals of the New York Academy of Sciences. 2000;916:533-539.