Insulin-responsive regions of the rat fatty acid synthase gene promoter

Siegmund S. Wolf; Gerhard Hofer; Karl-Friedrich Beck; Karim Roder; Michael Schweizer

doi:10.1006/bbrc.1994.2273

Insulin-responsive regions of the rat fatty acid synthase gene promoter

Siegmund S. Wolf
, Gerhard Hofer
, Karl-Friedrich Beck
, Karim Roder
, Michael Schweizer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Here we show that insulin may play a role in the diet-induced regulation of the rat fatty acid synthase (FAS; EC 2.3.1.85). Transient transfection of human and rat hepatoma cell lines with successively deleted FAS/CAT promoter fusion plasmids was used to determine the effect of insulin on FAS promoter activity. Our results indicate the existence of cis-acting insulin-responsive elements in the FAS promoter; the position of one of these is coincident with the position of a previously determined diet-induced DNAse I hypersensitive site (HS_i-1) at approximately -500 bp relative to the transcription start site of FAS mRNA.

Original language	English
Pages (from-to)	943-950
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	203
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1994.2273
Publication status	Published - 15 Sept 1994

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Insulin-responsive regions of the rat fatty acid synthase gene promoter",

abstract = "Here we show that insulin may play a role in the diet-induced regulation of the rat fatty acid synthase (FAS; EC 2.3.1.85). Transient transfection of human and rat hepatoma cell lines with successively deleted FAS/CAT promoter fusion plasmids was used to determine the effect of insulin on FAS promoter activity. Our results indicate the existence of cis-acting insulin-responsive elements in the FAS promoter; the position of one of these is coincident with the position of a previously determined diet-induced DNAse I hypersensitive site (HSi-1) at approximately -500 bp relative to the transcription start site of FAS mRNA.",

author = "Wolf, \{Siegmund S.\} and Gerhard Hofer and Karl-Friedrich Beck and Karim Roder and Michael Schweizer",

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T1 - Insulin-responsive regions of the rat fatty acid synthase gene promoter

AU - Wolf, Siegmund S.

AU - Hofer, Gerhard

AU - Beck, Karl-Friedrich

AU - Roder, Karim

AU - Schweizer, Michael

PY - 1994/9/15

Y1 - 1994/9/15

N2 - Here we show that insulin may play a role in the diet-induced regulation of the rat fatty acid synthase (FAS; EC 2.3.1.85). Transient transfection of human and rat hepatoma cell lines with successively deleted FAS/CAT promoter fusion plasmids was used to determine the effect of insulin on FAS promoter activity. Our results indicate the existence of cis-acting insulin-responsive elements in the FAS promoter; the position of one of these is coincident with the position of a previously determined diet-induced DNAse I hypersensitive site (HSi-1) at approximately -500 bp relative to the transcription start site of FAS mRNA.

AB - Here we show that insulin may play a role in the diet-induced regulation of the rat fatty acid synthase (FAS; EC 2.3.1.85). Transient transfection of human and rat hepatoma cell lines with successively deleted FAS/CAT promoter fusion plasmids was used to determine the effect of insulin on FAS promoter activity. Our results indicate the existence of cis-acting insulin-responsive elements in the FAS promoter; the position of one of these is coincident with the position of a previously determined diet-induced DNAse I hypersensitive site (HSi-1) at approximately -500 bp relative to the transcription start site of FAS mRNA.

UR - https://www.scopus.com/pages/publications/0028048793

U2 - 10.1006/bbrc.1994.2273

DO - 10.1006/bbrc.1994.2273

M3 - Article

AN - SCOPUS:0028048793

SN - 0006-291X

VL - 203

SP - 943

EP - 950

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Insulin-responsive regions of the rat fatty acid synthase gene promoter

Abstract

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