Ingestion of metal-nanoparticle contaminated food disrupts endogenous microbiota in zebrafish (Danio rerio)

Daniel L. Merrifield, Benjamin J. Shaw, Glenn M. Harper, Imad P. Saoud, Simon J. Davies, Richard D. Handy, Theodore B. Henry

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    63 Citations (Scopus)

    Abstract

    Nanoparticles (NPs) can be ingested by organisms, and NPs with antimicrobial properties may disrupt beneficial endogenous microbial communities and affect organism health. Zebrafish were fed diets containing Cu-NPs or Ag-NPs (500 mg kg(-1) food), or an appropriate control for 14 d. Intestinal epithelium integrity was examined by transmission electron microscopy, and microbial community structure within the intestine was assessed by denaturing gradient gel electrophoresis (DGGE) of partial 16S rRNA. No lesions were observed in intestinal epithelia; however, presence of NPs in diets changed intestinal microbial community structure. In particular, some beneficial bacterial strains (e.g., Cetobacterium somerae) were suppressed to non-detectable levels by Cu-NP exposure, and two unidentified bacterial clones from the Firmicutes phylum were sensitive (not detected) to Cu, but were present in Ag and control fish. Unique changes in zebrafish microbiome caused by exposure to Ag-NP and Cu-NP indicate that NP ingestion could affect digestive system function and organism health. (c) 2012 Elsevier Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)157-163
    Number of pages7
    JournalEnvironmental Pollution
    Volume174
    DOIs
    Publication statusPublished - Mar 2013

    Keywords

    • ESCHERICHIA-COLI
    • ONCORHYNCHUS-MYKISS
    • SILVER NANOPARTICLES
    • CARBON NANOTUBES
    • RAINBOW-TROUT
    • COPPER NANOPARTICLES
    • Nanotoxicology
    • Nanoparticles
    • Zebrafish
    • TITANIUM-DIOXIDE NANOPARTICLES
    • GUT MICROBIOTA
    • Endogenous microbiota
    • Intestinal epithelium integrity
    • ANTIBACTERIAL ACTIVITY
    • ANTIMICROBIAL ACTIVITY

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