Influence of carbon dioxide on the surface characteristics and adherence potential of coagulase-negative staphylococci

Stephen P. Denyer, Martyn C. Davies, J. A. Evans, Roger G. Finch, David George Emslie Smith, Mark H. Wilcox, Paul Williams

Research output: Contribution to journalArticle

Abstract

Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the surface chemistries of cells grown in the two atmospheres, as determined by X-ray photoelectron spectroscopy and changes in the cell wall protein profile. The growth atmosphere also influenced the adherence potential toward polystyrene and silicone in a proportion of strains examined. Thus, gaseous conditions can profoundly influence the nature of the staphylococcal surface, and this should be considered in any in vitro study of in vivo behavior.

Original languageEnglish
Pages (from-to)1813-1817
Number of pages5
JournalJournal of Clinical Microbiology
Volume28
Issue number8
Publication statusPublished - Aug 1990

Keywords

  • Bacterial Adhesion
  • Carbon Dioxide
  • Humans
  • Oxygen
  • Peritoneal Dialysis, Continuous Ambulatory
  • Polystyrenes
  • Silicones
  • Staphylococcus epidermidis
  • Surface Properties

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