TY - JOUR
T1 - In vivo FLIM-FRET measurements of recombinant proteins expressed in filamentous fungi
AU - Altenbach, Kirsten
AU - Duncan, Rory R.
AU - Valkonen, Mari
PY - 2009/8
Y1 - 2009/8
N2 - Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of Förster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.
AB - Fluorescence imaging techniques are extremely powerful tools in cell biology, providing valuable insights into the structure and function of biomolecules in their native environments. In particular, the use of Förster resonance energy transfer (FRET) has become increasingly important to obtain information on interactions on the nanoscale, in turn providing insights into molecular behaviour inside living cells. This review describes the basic principles of FRET and fluorescence lifetime imaging microscopy (FLIM) and their application in analyses of protein interactions inside living fungal cells.
U2 - 10.1016/j.fbr.2009.12.002
DO - 10.1016/j.fbr.2009.12.002
M3 - Article
SN - 1749-4613
VL - 23
SP - 67
EP - 71
JO - Fungal Biology Reviews
JF - Fungal Biology Reviews
IS - 3
ER -