In vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response

Nadia Ucciferri, Eva-Marie Collnot, Birgit Katja Gaiser, Annalisa Tirella, Vicki Stone, Claudio Domenici, Claus-Michael Lehr, Arti Ahluwalia

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    After passage through biological barriers, nanomaterials inevitably end up in contact with the vascular endothelium and can induce cardiovascular damage. In this study the toxicity and sublethal effects of 6 types of nanoparticle, including 4 of industrial and biomedical importance, on human endothelial cells was investigated using different in vitro assays. The results show that all the particles investigated induce some level of damage to the cells and that silver particles were most toxic, followed by titanium dioxide. Furthermore endothelial cells were shown to be more susceptible when exposed to silver nanoparticles under flow conditions in a bioreactor. The study underlines that although simple in vitro tests are useful to screen compounds and to identify the type of effect induced on cells, they may not be sufficient to define safe exposure limits. Therefore, once initial toxicity screening has been conducted on nanomaterials, it is necessary to develop more physiologically relevant in vitro models to better understand how nanomaterials can impact on human health.
    Original languageEnglish
    Pages (from-to)697-708
    Number of pages12
    JournalNanotoxicology
    Volume8
    Issue number6
    Early online date3 Sep 2013
    DOIs
    Publication statusPublished - 2014

    Keywords

    • nanoparticles
    • nanomaterials
    • toxicology
    • nanotoxicology
    • endothelial cells
    • bio-engineering
    • silver nanoparticles

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