TY - JOUR
T1 - Improved DNA vaccination by skin-targeted delivery using dry-coated densely-packed microprojection arrays
AU - Chen, Xianfeng
AU - Kask, Angela Shaulov
AU - Crichton, Michael L.
AU - McNeilly, Celia
AU - Yukiko, Sally
AU - Dong, Lichun
AU - Marshak, Joshua O.
AU - Jarrahian, Courtney
AU - Fernando, Germain J.P.
AU - Chen, Dexiang
AU - Koelle, David M.
AU - Kendall, Mark A.F.
PY - 2010/12/20
Y1 - 2010/12/20
N2 - HSV-2-gD2 DNA vaccine was precisely delivered to immunologically sensitive regions of the skin epithelia using dry-coated microprojection arrays. These arrays delivered a vaccine payload to the epidermis and the upper dermis of mouse skin. Immunomicroscopy results showed that, in 43 ± 5% of microprojection delivery sites, the DNA vaccine was delivered to contact with professional antigen presenting cells in the epidermal layer. Associated with this efficient delivery of the vaccine into the vicinity of the professional antigen presenting cells, we achieved superior antibody responses and statistically equal protection rate against an HSV-2 virus challenge, when compared with the mice immunized with intramuscular injection using needle and syringe, but with less than 1/10th of the delivered antigen.
AB - HSV-2-gD2 DNA vaccine was precisely delivered to immunologically sensitive regions of the skin epithelia using dry-coated microprojection arrays. These arrays delivered a vaccine payload to the epidermis and the upper dermis of mouse skin. Immunomicroscopy results showed that, in 43 ± 5% of microprojection delivery sites, the DNA vaccine was delivered to contact with professional antigen presenting cells in the epidermal layer. Associated with this efficient delivery of the vaccine into the vicinity of the professional antigen presenting cells, we achieved superior antibody responses and statistically equal protection rate against an HSV-2 virus challenge, when compared with the mice immunized with intramuscular injection using needle and syringe, but with less than 1/10th of the delivered antigen.
KW - DNA vaccine
KW - Microprojection
KW - Skin vaccine delivery
UR - http://www.scopus.com/inward/record.url?scp=78649802326&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2010.09.001
DO - 10.1016/j.jconrel.2010.09.001
M3 - Article
C2 - 20850487
AN - SCOPUS:78649802326
SN - 0168-3659
VL - 148
SP - 327
EP - 333
JO - Journal of Controlled Release
JF - Journal of Controlled Release
IS - 3
ER -
