Impact of microfluidic processing on bacterial ribonucleic acid expression

Senthil Kumar Gandi; David Watson; Maïwenn Kersaudy-Kerhoas; Marc P Y Desmulliez; Till Bachmann; Helen Bridle

doi:10.1063/1.4921819

Impact of microfluidic processing on bacterial ribonucleic acid expression

Senthil Kumar Gandi, David Watson, Maïwenn Kersaudy-Kerhoas, Marc P Y Desmulliez, Till Bachmann, Helen Bridle^*

^*Corresponding author for this work

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Abstract

Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A critical requirement for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered via microfluidic sample processing. This article investigates the impact of the use of microfluidics upon RNA expression of bacteria isolated from blood, a key step towards proving the suitability of such systems for further development.

Original language	English
Article number	031102
Journal	Biomicrofluidics
Volume	9
Issue number	3
DOIs	https://doi.org/10.1063/1.4921819
Publication status	Published - May 2015

ASJC Scopus subject areas

Genetics
Molecular Biology
Condensed Matter Physics
General Materials Science
Physical and Theoretical Chemistry

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10.1063/1.4921819

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© 2015 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Biomicrofluidics 9, 031102 (2015) and may be found at http://dx.doi.org/10.1063/1.4921819
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title = "Impact of microfluidic processing on bacterial ribonucleic acid expression",

abstract = "Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A critical requirement for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered via microfluidic sample processing. This article investigates the impact of the use of microfluidics upon RNA expression of bacteria isolated from blood, a key step towards proving the suitability of such systems for further development.",

author = "Gandi, {Senthil Kumar} and David Watson and Ma{\"i}wenn Kersaudy-Kerhoas and Desmulliez, {Marc P Y} and Till Bachmann and Helen Bridle",

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AU - Bachmann, Till

AU - Bridle, Helen

N1 - "The authors would like to acknowledge funding from the MRC Confidence in Concept scheme, MRC/CIC/002. H.B. and M.K.K. would like to acknowledge the Royal Academy of Engineering (RAEng)/EPSRC and RAEng, respectively, for their research fellowships."

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AB - Bacterial transcriptomics is widely used to investigate gene regulation, bacterial susceptibility to antibiotics, host-pathogen interactions, and pathogenesis. Transcriptomics is crucially dependent on suitable methods to isolate and detect bacterial RNA. Microfluidics offer ways of creating integrated point-of-care systems, analysing a sample from preparation, and RNA isolation to detection. A critical requirement for on-chip diagnostics to deliver on their promise is that mRNA expression is not altered via microfluidic sample processing. This article investigates the impact of the use of microfluidics upon RNA expression of bacteria isolated from blood, a key step towards proving the suitability of such systems for further development.

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Impact of microfluidic processing on bacterial ribonucleic acid expression

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Maïwenn Kersaudy-Kerhoas

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Impact of microfluidic processing on bacterial ribonucleic acid expression

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Maïwenn Kersaudy-Kerhoas

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