Imaging flow cytometry with femtosecond laser-micromachined glass microfluidic channels

Veerendra Kalyan Jagannadh, Mark Donald Mackenzie, Parama Pal, Ajoy K. Kar, Sai Siva Gorthi

Research output: Contribution to journalArticle

16 Citations (Scopus)
186 Downloads (Pure)

Abstract

Microfluidic/optofluidic microscopy is a versatile modality for imaging and analyzing properties of cells/particles while they are in flow. In this paper, we demonstrate the integration of fused silica microfluidics fabricated using femtosecond laser machining into optofluidic imaging systems. By using glass for the sample stage of our microscope, we have exploited its superior optical quality for imaging and bio-compatibility. By integrating these glass microfluidic devices into a custom-built bright field microscope, we have been able to image red blood cells in flow with high-throughputs and good fidelity. In addition, we also demonstrate imaging as well as detection of fluorescent beads with these microfluidic devices.

Original languageEnglish
Article number6990492
JournalIEEE Journal of Selected Topics in Quantum Electronics
Volume21
Issue number4
DOIs
Publication statusPublished - Jul 2015

Keywords

  • Glass microfluidics
  • High-throughput imaging
  • Microfluidic/ optofluidic imaging
  • Ultra-fast laser inscription

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

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