In vitro antagonistic activities of Lactobacillus spp. against Brachyspira hyodysenteriae and Brachyspira pilosicoli

Marion Bernardeau, Micheline Gueguen, David George Emslie Smith, Enrique Corona-Barrera, Jean Paul Vernoux

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    The sensitivity of Brachyspira hyodysenteriae and Brachyspira pilosicoli, respectively the causative agents of Swine Dysentery and Porcine Intestinal Spirochaetosis to two probiotic Lactobacillus strains, L. rhamnosus CNCM-I-3698 and L. farciminis CNCM-I-3699 was studied through viability, motility and coaggregation assays. The cell-free supernatant of these lactobacilli contains lactic acid, that is stressful for Brachyspira (leading to the formation of spherical bodies), and lethal. It was demonstrated for the first time the in vitro coaggregation properties of two probiotic Lactobacillus strains (active or heat-treated) with two pathogenic strains of Brachyspira, leading to (1) trapping of spirochaetal cells in a physical network as demonstrated by SEM; (2) inhibition of the motility of Brachyspira. Such in vitro studies should encourage in vivo studies in animal model to evaluate the potential of the use of probiotic lactobacilli through a feeding strategy for the prevention of B. hyodysenteriae and B. pilosicoli.

    Original languageEnglish
    Pages (from-to)184-190
    Number of pages7
    JournalVeterinary Microbiology
    Volume138
    Issue number1-2
    DOIs
    Publication statusPublished - 2 Jul 2009

    Keywords

    • Animals
    • Brachyspira
    • Brachyspira hyodysenteriae
    • Cell Movement
    • Cell Survival
    • Diarrhea
    • Lactobacillus
    • Microbial Sensitivity Tests
    • Probiotics
    • Swine
    • Swine Diseases

    Fingerprint Dive into the research topics of '<i>In vitro</i> antagonistic activities of <i>Lactobacillus</i> spp. against <i>Brachyspira hyodysenteriae</i> and <i>Brachyspira pilosicoli</i>'. Together they form a unique fingerprint.

  • Cite this