Identification of grapevine MLO gene candidates involved in susceptibility to powdery mildew

Angela Feechan, Angelica M. Jermakow, Laurent Torregrosa, Ralph Panstruga, Ian B. Dry

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

The European cultivated grapevine, Vitis vinifera L., is a host for the powdery mildew pathogen Erisyphe necator, which is the most economically important fungal disease of viticulture. MLO proteins mediate powdery mildew susceptibility in the model plant species Arabidopsis and the crop plants barley and tomato. Seven VvMLO cDNA sequences were isolated from grapevine and were subsequently identified as part of a 17 member VvMLO gene family within the V. vinifera genome. Phylogenetic analysis of the 17 VvMLO genes in the grape genome indicated that the proteins they encode fall into six distinct clades. The expression of representative VvMLOs from each clade were analysed in a range of grape tissues, as well as in response to a range of biotic and abiotic factors. The VvMLOs investigated have unique, but overlapping tissue expression patterns. Expression analysis of VvMLO genes following E. necator infection identified four upregulated VvMLOs which are orthologous to the Arabidopsis AtMLO2, AtMLO6 and AtMLO12 and tomato SlMLO1 genes required for powdery mildew susceptibility. This suggests a degree of functional redundancy between the proteins encoded by these genes in terms of susceptibility to powdery mildew, and, as such, represent potential targets for modification to generate powdery mildew resistant grapevines.

Original languageEnglish
Pages (from-to)1255-1266
Number of pages12
JournalFunctional Plant Biology
Volume35
Issue number12
DOIs
Publication statusPublished - 16 Dec 2008

Keywords

  • Erysiphe necator
  • Grapevine
  • MLO
  • Powdery mildew

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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