Identification of cDNAs induced by the organophosphate trichlorphon in the parasitic copepod Lepeophtheirus salmonis (Copepoda; Caligidae)

Thomas K. Walsh; Alastair R. Lyndon; Derek J. Jamieson

doi:10.1016/j.pestbp.2006.08.008

Identification of cDNAs induced by the organophosphate trichlorphon in the parasitic copepod Lepeophtheirus salmonis (Copepoda; Caligidae)

Thomas K. Walsh, Alastair R. Lyndon, Derek J. Jamieson

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Lepeophtheirus salmonis is a copepod ectoparasite of wild and farmed salmonids and can cause considerable damage to infected fish. We have examined the effect of the organophosphate trichlorphon, which was one of the early treatments for sea lice as Neguvon^® on L. salmonis. Suppression subtractive hybridisation was used to prepare a cDNA library enriched for cDNAs expressed in response to the organophosphate trichlorophon and using this technique a total of 33 cDNAs were found to be differentially regulated in response to organophosphate exposure. Sequence analysis of the cDNAs revealed that many were involved in cellular stress responses and or neurotoxicity. The expression of two of these cDNAs was confirmed to be up-regulated in response to trichlorophon. © 2006 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	26-30
Number of pages	5
Journal	Pesticide Biochemistry and Physiology
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/j.pestbp.2006.08.008
Publication status	Published - May 2007

Keywords

cDNA
Copepoda
Lepeophtheirus salmonis
Organophosphates
Suppression subtractive hybridisation
Xenobiotics

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10.1016/j.pestbp.2006.08.008

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title = "Identification of cDNAs induced by the organophosphate trichlorphon in the parasitic copepod Lepeophtheirus salmonis (Copepoda; Caligidae)",

abstract = "Lepeophtheirus salmonis is a copepod ectoparasite of wild and farmed salmonids and can cause considerable damage to infected fish. We have examined the effect of the organophosphate trichlorphon, which was one of the early treatments for sea lice as Neguvon{\textregistered} on L. salmonis. Suppression subtractive hybridisation was used to prepare a cDNA library enriched for cDNAs expressed in response to the organophosphate trichlorophon and using this technique a total of 33 cDNAs were found to be differentially regulated in response to organophosphate exposure. Sequence analysis of the cDNAs revealed that many were involved in cellular stress responses and or neurotoxicity. The expression of two of these cDNAs was confirmed to be up-regulated in response to trichlorophon. {\textcopyright} 2006 Elsevier Inc. All rights reserved.",

keywords = "cDNA, Copepoda, Lepeophtheirus salmonis, Organophosphates, Suppression subtractive hybridisation, Xenobiotics",

author = "Walsh, {Thomas K.} and Lyndon, {Alastair R.} and Jamieson, {Derek J.}",

year = "2007",

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TY - JOUR

T1 - Identification of cDNAs induced by the organophosphate trichlorphon in the parasitic copepod Lepeophtheirus salmonis (Copepoda; Caligidae)

AU - Walsh, Thomas K.

AU - Lyndon, Alastair R.

AU - Jamieson, Derek J.

PY - 2007/5

Y1 - 2007/5

N2 - Lepeophtheirus salmonis is a copepod ectoparasite of wild and farmed salmonids and can cause considerable damage to infected fish. We have examined the effect of the organophosphate trichlorphon, which was one of the early treatments for sea lice as Neguvon® on L. salmonis. Suppression subtractive hybridisation was used to prepare a cDNA library enriched for cDNAs expressed in response to the organophosphate trichlorophon and using this technique a total of 33 cDNAs were found to be differentially regulated in response to organophosphate exposure. Sequence analysis of the cDNAs revealed that many were involved in cellular stress responses and or neurotoxicity. The expression of two of these cDNAs was confirmed to be up-regulated in response to trichlorophon. © 2006 Elsevier Inc. All rights reserved.

AB - Lepeophtheirus salmonis is a copepod ectoparasite of wild and farmed salmonids and can cause considerable damage to infected fish. We have examined the effect of the organophosphate trichlorphon, which was one of the early treatments for sea lice as Neguvon® on L. salmonis. Suppression subtractive hybridisation was used to prepare a cDNA library enriched for cDNAs expressed in response to the organophosphate trichlorophon and using this technique a total of 33 cDNAs were found to be differentially regulated in response to organophosphate exposure. Sequence analysis of the cDNAs revealed that many were involved in cellular stress responses and or neurotoxicity. The expression of two of these cDNAs was confirmed to be up-regulated in response to trichlorophon. © 2006 Elsevier Inc. All rights reserved.

KW - cDNA

KW - Copepoda

KW - Lepeophtheirus salmonis

KW - Organophosphates

KW - Suppression subtractive hybridisation

KW - Xenobiotics

U2 - 10.1016/j.pestbp.2006.08.008

DO - 10.1016/j.pestbp.2006.08.008

M3 - Article

SN - 0048-3575

VL - 88

SP - 26

EP - 30

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

IS - 1

ER -

Identification of cDNAs induced by the organophosphate trichlorphon in the parasitic copepod Lepeophtheirus salmonis (Copepoda; Caligidae)

Abstract

Keywords

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