Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis

Johanna Jefferies, Leena Nieminen, Lea-Ann Kirkham, Calum Johnston, Andrew Smith, Tim J. Mitchell

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mitilysin extracellularly. Certain alleles of mitilysin are not recognized by a monoclonal antibody raised to the related toxin pneumolysin. Based on enzyme-linked immunosorbent assay and neutralization assay results, one isolate of S. mitis may produce a further hemolytic toxin in addition to mitilysin. As genetic exchange is known to occur between S. mitis and Streptococcus pneumoniae, this finding may have implications for the development of vaccines or therapies for pneumococcal disease that are based on pneumolysin.

Original languageEnglish
Pages (from-to)627-632
Number of pages6
JournalJournal of Bacteriology
Volume189
Issue number2
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Amino Acid Sequence
  • Bacterial Proteins/genetics
  • Bacterial Toxins/genetics
  • Blotting, Western
  • Cholesterol/metabolism
  • DNA, Bacterial/chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Streptococcus mitis/genetics
  • Streptococcus pneumoniae/genetics
  • Streptolysins/genetics

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