Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants

Tsuyoshi Mizoguchi, Kazuya Ichimura, Kenji Irie, Peter Morris, Jérôme Giraudat, Kunihiro Matsumoto, Kazuo Shinozaki

    Research output: Contribution to journalArticle

    Abstract

    A possible MAP kinase (MAPK) cascade of Arabidopsis thaliana was identified on the basis of both yeast 2-hybrid analysis and complementation analysis of yeast mutants. Specific protein-protein interactions between ATMPK4 (a MAPK) and MEK1 (a MAPKK) and interactions between MEK1 and ATMEKK1 (a MAPKKK) were detected by using the 2-hybrid system. A growth defect of the yeast mpk1? mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N-terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2? mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants. Copyright (C) 1998 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)56-60
    Number of pages5
    JournalFEBS Letters
    Volume437
    Issue number1-2
    DOIs
    Publication statusPublished - 16 Oct 1998

    Keywords

    • Arabidopsis thaliana
    • Mitogen-activated protein kinase cascade
    • trans-Complementation
    • Yeast two-hybrid

    Fingerprint Dive into the research topics of 'Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants'. Together they form a unique fingerprint.

  • Cite this