Identification of a glucose–mannose phosphotransferase system in Clostridium beijerinckii

Mohemed Essalem Emhemed Essalem, Wilfrid James Mitchell

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Effective uptake of fermentable substrates is a fundamentally important aspect of any fermentation process. The solventogenic bacterium Clostridium beijerinckii is noted for its ability to ferment a wide range of carbohydrates, yet few of its sugar transport systems have been characterized. In common with other anaerobes, C. beijerinckii shows a marked dependence on the PEP-dependent phosphotransferase system (PTS) for sugar accumulation. In this study, the gene cbe0751 encoding the sugar-specific domains of a phosphotransferase belonging to the glucose family was cloned into an Escherichia coli strain lacking the ability to take up and phosphorylate glucose. Transformants gained ability to ferment glucose, and also mannose, and further analysis of a selected transformant demonstrated that it could take up and phosphorylate glucose, confirming that cbe0751 encodes a glucose PTS which also recognizes mannose as a substrate. RT-PCR analysis showed that cbe0751 was expressed in cultures grown on both substrates, but also to varying extents during growth on some other carbon sources. Although analogue inhibition studies suggested that Cbe0751 is not the only glucose PTS in C. beijerinckii, this system should nevertheless be regarded as a potential target for metabolic engineering to generate a strain showing improved sugar fermentation properties.

Original languageEnglish
Article numberfnw053
JournalFEMS Microbiology Letters
Volume363
Issue number8
DOIs
Publication statusPublished - 31 Mar 2016

Keywords

  • Acetone butanol
  • Clostridium
  • Fermentation
  • Glucose
  • Mannose
  • Phosphotransferase

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

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