Identification and typing of vibrio anguillarum: A comparison of different methods

B. Austin, M. Alsina, D. A. Austin, A. R. Blanch, F. Grimont, P. A D Grimont, J. Jofre, S. Koblavi, J. L. Larsen, K. Pedersen, T. Tiainen, L. Verdonck, J. Swings

    Research output: Contribution to journalArticle

    Abstract

    The majority (91%) of 260 isolates initially identified as Vibrio anguillarum, that were obtained from a wide range of hosts, habitats and geographical locations, were recovered in a single cluster based on the ribotype and were pathogenic to Atlantic salmon. A significant proportion of isolates (78% of the total) were allocated to 15 serogroups (O1-O10 and five previously undescribed groups referred to as VaNT1, VaNT2, VaNT4, NaNT5 and VaNT7). A minority of isolates (6%) reacted with more than one antiserum or were self-agglutinating, and the remainder did not react with any of the antisera tested. Good correlation was noted between serogroups and lipopolysaccharide profiles, particularly with respect to isolates belonging to serogroups O1, O2 and O4-O10. Plasmids were recognized in some serogroups, especially O1, which contained the 67 kb plasmid associated with virulence. However, the 19 profiles based on outer membrane protein patterns did not correspond to the results obtained with the other typing methods. Generally, the isolates were heterogeneous in their biochemical characteristics; 117 profiles were obtained with the API 20E system, and 9 and 32 clusters recognised from the results of BIOLOG fingerprinting and Biotype-100 biotyping methods, respectively. Three dominant clusters were defined from fatty acid methyl esters profiles.

    Original languageEnglish
    Pages (from-to)285-302
    Number of pages18
    JournalSystematic and Applied Microbiology
    Volume18
    Issue number2
    Publication statusPublished - 1995

    Keywords

    • Fish isolates
    • Fish pathogens
    • Identification
    • Typing methods
    • Vibrio

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    Austin, B., Alsina, M., Austin, D. A., Blanch, A. R., Grimont, F., Grimont, P. A. D., Jofre, J., Koblavi, S., Larsen, J. L., Pedersen, K., Tiainen, T., Verdonck, L., & Swings, J. (1995). Identification and typing of vibrio anguillarum: A comparison of different methods. Systematic and Applied Microbiology, 18(2), 285-302.