Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells

Peter Burton, David R. Adams, Achamma Abraham, Robert W. Allcock, Zhong Jiang, Angela McCahill, Jane Gilmour, John McAbney, Nicole M. Kane, George S. Baillie, Fergus R. McKenzie, Andrew H. Baker, Miles D. Houslay, Joanne C. Mountford, Graeme Milligan

Research output: Contribution to journalArticle

Abstract

hESCs (human embryonic stem cells) offer great potential for pharmaceutical research and development and, potentially, for therapeutic use. However, improvements in cell culture are urgently required to allow the scalable production of large numbers of cells that maintain pluripotency. Supplementing feeder-free conditions with either EHNA [erythro-9-(2-hydroxy-3-nonyl)adenine] or readily synthesized analogues of this compound maintains hESC pluripotency in the absence of exogenous cytokines. When the hESC lines SA121 or SA461 were maintained in feeder-free conditions with EHNA they displayed no reduction in stem-cell-associated markers such as Nanog, Oct4 (octamer-binding protein 4) and SSEA4 (stage-specific embryonic antigen 4) when compared with cells maintained in full feeder-free conditions that included exogenously added bFGF (basic fibroblast growth factor). Spontaneous differentiation was reversibly suppressed by the addition of EHNA, but EHNA did not limit efficient spontaneous or directed differentiation following its removal. We conclude that EHNA or related compounds offers a viable alternative to exogenous cytokine addition in maintaining hESC cultures in a pluripotent state and might be a particularly useful replacement for bFGF for large-scale or GMP (good manufacturing practice)-compliant processes. ©The Authors.

Original languageEnglish
Pages (from-to)1058-1061
Number of pages4
JournalBiochemical Society Transactions
Volume38
Issue number4
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Adenosine deaminase
  • Human embryonic stem cell
  • Phosphodiesterase
  • Pluripotency

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    Burton, P., Adams, D. R., Abraham, A., Allcock, R. W., Jiang, Z., McCahill, A., Gilmour, J., McAbney, J., Kane, N. M., Baillie, G. S., McKenzie, F. R., Baker, A. H., Houslay, M. D., Mountford, J. C., & Milligan, G. (2010). Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells. Biochemical Society Transactions, 38(4), 1058-1061. https://doi.org/10.1042/BST0381058