Identification and characterisation of a homologue of p64 in rat tissues

Steven Howell, Rory Duncan, Richard H. Ashley

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Previous work has suggested that the gene encoding p64, a component of a bovine kidney intracellular chloride channel, may be a member of a gene family. We have raised a polyclonal antibody to an E. coli fusion protein which has sequence similarity to p64. Immunoblotting detected a protein in rat brain, kidney, liver and lung. In rat brain, the protein was enriched in cerebellar microsomal membranes. Western blot analyses of denaturing and blue native polyacrylamide gels indicated that the protein is a single non-disulphide-linked polypeptide chain with an apparent M(r) of 43 kDa that contributes to a native protein complex with an apparent M(r) of 130 kDa.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalFEBS Letters
Volume390
Issue number2
DOIs
Publication statusPublished - 22 Jul 1996

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain
  • Cattle
  • Chloride Channels
  • DNA Primers
  • DNA, Complementary
  • Kidney
  • Liver
  • Lung
  • Molecular Sequence Data
  • Molecular Weight
  • Multigene Family
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • Sequence Homology, Amino Acid
  • Tissue Distribution

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