Identification and characterisation of a homologue of p64 in rat tissues

Steven Howell; Rory Duncan; Richard H. Ashley

doi:10.1016/0014-5793(96)00676-X

Identification and characterisation of a homologue of p64 in rat tissues

Steven Howell, Rory Duncan, Richard H. Ashley

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Previous work has suggested that the gene encoding p64, a component of a bovine kidney intracellular chloride channel, may be a member of a gene family. We have raised a polyclonal antibody to an E. coli fusion protein which has sequence similarity to p64. Immunoblotting detected a protein in rat brain, kidney, liver and lung. In rat brain, the protein was enriched in cerebellar microsomal membranes. Western blot analyses of denaturing and blue native polyacrylamide gels indicated that the protein is a single non-disulphide-linked polypeptide chain with an apparent M(r) of 43 kDa that contributes to a native protein complex with an apparent M(r) of 130 kDa.

Original language	English
Pages (from-to)	207-210
Number of pages	4
Journal	FEBS Letters
Volume	390
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(96)00676-X
Publication status	Published - 22 Jul 1996

Keywords

Amino Acid Sequence
Animals
Base Sequence
Brain
Cattle
Chloride Channels
DNA Primers
DNA, Complementary
Kidney
Liver
Lung
Molecular Sequence Data
Molecular Weight
Multigene Family
Rats
Rats, Sprague-Dawley
Recombinant Fusion Proteins
Sequence Homology, Amino Acid
Tissue Distribution

Access to Document

10.1016/0014-5793(96)00676-X

Cite this

@article{52f82ce6dcba4a4ab0ab6dab0fb14236,

title = "Identification and characterisation of a homologue of p64 in rat tissues",

abstract = "Previous work has suggested that the gene encoding p64, a component of a bovine kidney intracellular chloride channel, may be a member of a gene family. We have raised a polyclonal antibody to an E. coli fusion protein which has sequence similarity to p64. Immunoblotting detected a protein in rat brain, kidney, liver and lung. In rat brain, the protein was enriched in cerebellar microsomal membranes. Western blot analyses of denaturing and blue native polyacrylamide gels indicated that the protein is a single non-disulphide-linked polypeptide chain with an apparent M(r) of 43 kDa that contributes to a native protein complex with an apparent M(r) of 130 kDa.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Brain, Cattle, Chloride Channels, DNA Primers, DNA, Complementary, Kidney, Liver, Lung, Molecular Sequence Data, Molecular Weight, Multigene Family, Rats, Rats, Sprague-Dawley, Recombinant Fusion Proteins, Sequence Homology, Amino Acid, Tissue Distribution",

author = "Steven Howell and Rory Duncan and Ashley, {Richard H.}",

year = "1996",

month = jul,

day = "22",

doi = "10.1016/0014-5793(96)00676-X",

language = "English",

volume = "390",

pages = "207--210",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Identification and characterisation of a homologue of p64 in rat tissues

AU - Howell, Steven

AU - Duncan, Rory

AU - Ashley, Richard H.

PY - 1996/7/22

Y1 - 1996/7/22

N2 - Previous work has suggested that the gene encoding p64, a component of a bovine kidney intracellular chloride channel, may be a member of a gene family. We have raised a polyclonal antibody to an E. coli fusion protein which has sequence similarity to p64. Immunoblotting detected a protein in rat brain, kidney, liver and lung. In rat brain, the protein was enriched in cerebellar microsomal membranes. Western blot analyses of denaturing and blue native polyacrylamide gels indicated that the protein is a single non-disulphide-linked polypeptide chain with an apparent M(r) of 43 kDa that contributes to a native protein complex with an apparent M(r) of 130 kDa.

AB - Previous work has suggested that the gene encoding p64, a component of a bovine kidney intracellular chloride channel, may be a member of a gene family. We have raised a polyclonal antibody to an E. coli fusion protein which has sequence similarity to p64. Immunoblotting detected a protein in rat brain, kidney, liver and lung. In rat brain, the protein was enriched in cerebellar microsomal membranes. Western blot analyses of denaturing and blue native polyacrylamide gels indicated that the protein is a single non-disulphide-linked polypeptide chain with an apparent M(r) of 43 kDa that contributes to a native protein complex with an apparent M(r) of 130 kDa.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Brain

KW - Cattle

KW - Chloride Channels

KW - DNA Primers

KW - DNA, Complementary

KW - Kidney

KW - Liver

KW - Lung

KW - Molecular Sequence Data

KW - Molecular Weight

KW - Multigene Family

KW - Rats

KW - Rats, Sprague-Dawley

KW - Recombinant Fusion Proteins

KW - Sequence Homology, Amino Acid

KW - Tissue Distribution

U2 - 10.1016/0014-5793(96)00676-X

DO - 10.1016/0014-5793(96)00676-X

M3 - Article

C2 - 8706861

SN - 0014-5793

VL - 390

SP - 207

EP - 210

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Identification and characterisation of a homologue of p64 in rat tissues

Abstract

Keywords

Access to Document

Fingerprint

Cite this