Campylobacter jejuni 81-176 forms distinct microcolonies on in vitro-infected human small intestinal tissue prior to biofilm formation

Graham Haddock, Margaret Mullin, Amanda MacCallum, Aileen E. Sherry, Laurence Tetley, Eleanor Watson, Mark P. Dagleish, David George Emslie Smith, Paul Everest

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Human small and large intestinal tissue was used to study the interaction of Campylobacter jejuni with its target tissue. The strain used for the study was 81-176 (+pVir). Tissue was processed for scanning and transmission electron microscopy, and by immunohistochemistry for light microscopy. Organisms adhered to the apical surface of ileal tissues at all time points in large numbers, in areas where mucus was present and in distinct groups. Microcolony formation was evident at 1-2 h, with bacteria adhering to mucus on the tissue surface and to each other by flagellar interaction. At later time points (3-4 h), biofilm formation on ileal tissue was evident. Flagellar mutants did not form microcolonies or biofilms in tissue. Few organisms were observed in colonic tissue, with organisms present but not as abundant as in the ileal tissue. This study shows that C. jejuni 81-176 can form microcolonies and biofilms on human intestinal tissue and that this may be an essential step in its ability to cause diarrhoea in man.

    Original languageEnglish
    Pages (from-to)3079-3084
    Number of pages6
    JournalMicrobiology
    Volume156
    Issue number10
    DOIs
    Publication statusPublished - Oct 2010

    Keywords

    • Bacterial Adhesion
    • Biofilms
    • Campylobacter jejuni
    • Humans
    • In Vitro Techniques
    • Intestine, Large
    • Intestine, Small
    • Microscopy, Electron, Scanning
    • Microscopy, Electron, Transmission

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