Human glioma tumors detection by a portable visible resonance Raman analyzer with a hand-held optical fiber probe

Yan Zhou*, Shengjia Zhang, Binlin Wu, Xinguang Yu, Gangge Cheng, Ke Zhu, Mingyue Zhao, Jichun Zheng, Liang Zhang, Mingqian Zhang, Qijun Liang, Yun Ii Go, Chunyuan Zhang, Cheng Hui Liu, Robert R. Alfano

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)
88 Downloads (Pure)


Based on Visible Resonance Raman (VRR) method, we have developed a novel label-free portable VRR LRR2000 Raman analyzer with a portable fiber-optic probe and used it for the classification of human gliomas ex vivo and for the analysis of changes in tumor chemical compositions in molecular level. The purpose of this study was to examine the performance of the LRR2000 Raman analyzer as an optical biopsy tool for detecting human brain tumors compared to the commercial laboratory HR800 and WITec300 micro confocal Raman spectroscopy instruments. As of 2018, a total 1,938 VRR spectra were collected using LRR2000, HR800 and WITec300 Raman system, ex vivo. Identification of the four grades of glioma tumors and control tissues was performed based on the characteristic native molecular fingerprints. LRR2000 demonstrated consistent diagnostic results with HR800 and WITec300 Raman systems. LRR2000 showed the advantages of high speed, convenience and low cost compared to the two confocal micro Raman systems. Using artificial intelligence (AI)-based analysis of part of the data, the cross-validated accuracy for identifying glioma tumors is ∼90% compared with gold standard histopathology examination.

Original languageEnglish
Title of host publicationBiomedical Vibrational Spectroscopy 2020
Subtitle of host publicationAdvances in Research and Industry
EditorsWolfgang Petrich, Zhiwei Huang
ISBN (Electronic)9781510632363
ISBN (Print)9781510632356
Publication statusPublished - 21 Feb 2020
EventSPIE BIOS 2020 - San Francisco, United States
Duration: 1 Feb 20206 Feb 2020

Publication series

NameProceedings of SPIE
ISSN (Print)1605-7422
ISSN (Electronic)2410-9045


ConferenceSPIE BIOS 2020
Country/TerritoryUnited States
CitySan Francisco


  • glioma tumor
  • handheld LRR2000 analyzer
  • human brain tissues
  • optical biopsy
  • Optical molecular Pathology
  • principle component analysis(PCA)
  • support vector machine (SVM)
  • visible resonance Raman (VRR)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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