Histopathological effects of waterborne copper nanoparticles and copper sulphate on the organs of rainbow trout (Oncorhynchus mykiss)

Genan A. Al-Bairuty, Benjamin J. Shaw, Richard D. Handy, Theodore B. Henry

    Research output: Contribution to journalArticle

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    Abstract

    It is unclear whether copper nanoparticles are more toxic than traditional forms of dissolved copper. This study aimed to describe the pathologies in gill, gut, liver, kidney, brain and muscle of juvenile rainbow trout, Oncorhynchus mykiss, exposed in triplicate to either a control (no added Cu), 20 or 100 mu gl(-1) of either dissolved Cu (as CuSO4) or Cu-NPs (mean primary particle size of 87 +/- 27 nm) in a semi-static waterborne exposure regime. Fish were sampled at days 0, 4, and 10 for histology. All treatments caused organ injuries, and the kinds of pathologies observed with Cu-NPs were broadly of the same type as CuSO4 including: hyperplasia, aneurisms, and necrosis in the secondary lamellae of the gills; swelling of goblet cells, necrosis in the mucosa layer and vacuole formation in the gut; hepatitis-like injury and cells with pyknotic nuclei in the liver; damage to the epithelium of some renal tubules and increased Bowman's space in the kidney. In the brain, some mild changes were observed in the nerve cell bodies in the telencephalon, alteration in the thickness of the mesencephalon layers, and enlargement of blood vessel on the ventral surface of the cerebellum. Changes in the proportional area of muscle fibres were observed in skeletal muscle. Overall the data showed that pathology from CuSO4 and Cu-NPs were of similar types, but there were some material-type effects in the severity or incidence of injuries with Cu-NPs causing more injury in the intestine, liver and brain than the equivalent concentration of CuSO4 by the end of the experiment, but in the gill and muscle CuSO4 caused more pathology. (c) 2012 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)104-115
    Number of pages12
    JournalAquatic Toxicology
    Volume126
    DOIs
    Publication statusPublished - 15 Jan 2013

    Keywords

    • FRESH-WATER
    • Brain injury
    • OREOCHROMIS-NILOTICUS
    • DIETARY COPPER
    • EXPOSURE
    • NANOMATERIALS
    • Gill pathology
    • TITANIUM-DIOXIDE NANOPARTICLES
    • Renal pathology
    • Hypoxia
    • CYPRINUS-CARPIO
    • Copper toxicity
    • OXIDATIVE STRESS
    • Fish
    • TOXICITY
    • NILE TILAPIA
    • Copper nanoparticles

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