Highly sensitive rapid fluorescence detection of protein residues on surgical instruments

Valeri I. Kovalev; James S. Barton; Patricia R. Richardson; Anita C. Jones

doi:10.1117/12.646137

Highly sensitive rapid fluorescence detection of protein residues on surgical instruments

Valeri I. Kovalev, James S. Barton, Patricia R. Richardson, Anita C. Jones

School of Engineering & Physical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

There is a risk of contamination of surgical instruments by infectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~100 zeptomoles/mm² with an area scan speed of ~20 cm²/s and for using the system to detect other agents of biomedical interest. A theoretical analysis and experimental measurements will be discussed.

Original language	English
Article number	60920J
Journal	Progress in Biomedical Optics and Imaging
Volume	6092
DOIs	https://doi.org/10.1117/12.646137
Publication status	Published - 2006
Event	Fiber Lasers III: Technology, Systems, and Applications - San Jose, CA, United States Duration: 23 Jan 2006 → 26 Jan 2006

Keywords

Laser-induced fluorescence
Optical diagnostics for medicine

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10.1117/12.646137

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title = "Highly sensitive rapid fluorescence detection of protein residues on surgical instruments",

abstract = "There is a risk of contamination of surgical instruments by infectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~100 zeptomoles/mm2 with an area scan speed of ~20 cm2/s and for using the system to detect other agents of biomedical interest. A theoretical analysis and experimental measurements will be discussed.",

keywords = "Laser-induced fluorescence, Optical diagnostics for medicine",

author = "Kovalev, {Valeri I.} and Barton, {James S.} and Richardson, {Patricia R.} and Jones, {Anita C.}",

year = "2006",

doi = "10.1117/12.646137",

language = "English",

volume = "6092",

journal = "Progress in Biomedical Optics and Imaging",

issn = "1605-7422",

publisher = "SPIE",

note = "Fiber Lasers III: Technology, Systems, and Applications ; Conference date: 23-01-2006 Through 26-01-2006",

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TY - JOUR

T1 - Highly sensitive rapid fluorescence detection of protein residues on surgical instruments

AU - Kovalev, Valeri I.

AU - Barton, James S.

AU - Richardson, Patricia R.

AU - Jones, Anita C.

PY - 2006

Y1 - 2006

N2 - There is a risk of contamination of surgical instruments by infectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~100 zeptomoles/mm2 with an area scan speed of ~20 cm2/s and for using the system to detect other agents of biomedical interest. A theoretical analysis and experimental measurements will be discussed.

AB - There is a risk of contamination of surgical instruments by infectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~100 zeptomoles/mm2 with an area scan speed of ~20 cm2/s and for using the system to detect other agents of biomedical interest. A theoretical analysis and experimental measurements will be discussed.

KW - Laser-induced fluorescence

KW - Optical diagnostics for medicine

U2 - 10.1117/12.646137

DO - 10.1117/12.646137

M3 - Article

SN - 1605-7422

VL - 6092

JO - Progress in Biomedical Optics and Imaging

JF - Progress in Biomedical Optics and Imaging

M1 - 60920J

T2 - Fiber Lasers III: Technology, Systems, and Applications

Y2 - 23 January 2006 through 26 January 2006

ER -

Highly sensitive rapid fluorescence detection of protein residues on surgical instruments

Abstract

Keywords

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