TY - JOUR
T1 - Highly sensitive rapid fluorescence detection of protein residues on surgical instruments
AU - Kovalev, Valeri I.
AU - Bartona, James S.
AU - Richardson, Patricia R.
AU - Jones, Anita C.
PY - 2006/7/1
Y1 - 2006/7/1
N2 - There is a risk of contamination of surgical instruments by infectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~10 attomole/cm2 with a scan speed of ~3-10 cm2/s of the test instrument's surface. A theoretical analysis and experimental measurements will be discussed. © 2006 IOP Publishing Ltd.
AB - There is a risk of contamination of surgical instruments by infectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~10 attomole/cm2 with a scan speed of ~3-10 cm2/s of the test instrument's surface. A theoretical analysis and experimental measurements will be discussed. © 2006 IOP Publishing Ltd.
UR - http://www.scopus.com/inward/record.url?scp=33747180899&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/45/1/027
DO - 10.1088/1742-6596/45/1/027
M3 - Article
SN - 1742-6588
VL - 45
SP - 201
EP - 206
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 027
ER -