TY - JOUR
T1 - Highly sensitive detection of NADH using electrochemiluminescent nanocomposites
AU - Devadoss, Anitha
AU - Dennany, Lynn
AU - Dickinson, Calum
AU - Keyes, Tia E.
AU - Forster, Robert J.
PY - 2012/6
Y1 - 2012/6
N2 - Electrodes, modified with [Ru(bpy)2PVP10]n(ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2′-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than for the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 μM with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection make these nanocomposite films attractive for biosensor development.
AB - Electrodes, modified with [Ru(bpy)2PVP10]n(ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2′-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than for the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 μM with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection make these nanocomposite films attractive for biosensor development.
U2 - 10.1016/j.elecom.2012.03.003
DO - 10.1016/j.elecom.2012.03.003
M3 - Article
SN - 1388-2481
VL - 19
SP - 43
EP - 45
JO - Electrochemistry Communications
JF - Electrochemistry Communications
ER -