High fidelity fibre-based physiological sensing deep in tissue

Tushar Choudhary; Michael George Tanner; Alicia Megia-Fernandez; Kerrianne Harrington; Harry A. C. Wood; Adam Marshall; Patricia Zhu; Sunay V. Chankeshwara; Debaditya Choudhury; Graham Monro; Muhammed Ucuncu; Fei Yu; Rory R. Duncan; Robert R. Thomson; Kevin Dhaliwal; Mark Bradley

doi:10.1038/s41598-019-44077-7

High fidelity fibre-based physiological sensing deep in tissue

Tushar Choudhary, Michael George Tanner, Alicia Megia-Fernandez, Kerrianne Harrington, Harry A. C. Wood, Adam Marshall, Patricia Zhu, Sunay V. Chankeshwara, Debaditya Choudhury, Graham Monro, Muhammed Ucuncu, Fei Yu, Rory R. Duncan, Robert R. Thomson, Kevin Dhaliwal, Mark Bradley

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Abstract

Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (± 0.02 pH units and ± 0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.

Original language	English
Article number	7713
Journal	Scientific Reports
Volume	9
DOIs	https://doi.org/10.1038/s41598-019-44077-7
Publication status	Published - 22 May 2019

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Choudhary, T., Tanner, M. G., Megia-Fernandez, A., Harrington, K., Wood, H. A. C., Marshall, A., Zhu, P., Chankeshwara, S. V., Choudhury, D., Monro, G., Ucuncu, M., Yu, F., Duncan, R. R., Thomson, R. R., Dhaliwal, K., & Bradley, M. (2019). High fidelity fibre-based physiological sensing deep in tissue. Scientific Reports, 9, Article 7713. https://doi.org/10.1038/s41598-019-44077-7

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abstract = "Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (± 0.02 pH units and ± 0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.",

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AU - Harrington, Kerrianne

AU - Wood, Harry A. C.

AU - Marshall, Adam

AU - Zhu, Patricia

AU - Chankeshwara, Sunay V.

AU - Choudhury, Debaditya

AU - Monro, Graham

AU - Ucuncu, Muhammed

AU - Yu, Fei

AU - Duncan, Rory R.

AU - Thomson, Robert R.

AU - Dhaliwal, Kevin

AU - Bradley, Mark

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AB - Physiological sensing deep in tissue remains a clinical challenge. Here a flexible miniaturised sensing optrode providing a platform to perform minimally invasive in vivo in situ measurements is reported. Silica microspheres covalently coupled with a high density of ratiometrically configured fluorophores were deposited into etched pits on the distal end of a 150 µm diameter multicore optical fibre. With this platform, photonic measurements of pH and oxygen concentration with high precision in the distal alveolar space of the lung are reported. We demonstrated the phenomenon that high-density deposition of carboxyfluorescein covalently coupled to silica microspheres shows an inverse shift in fluorescence in response to varying pH. This platform delivered fast and accurate measurements (± 0.02 pH units and ± 0.6 mg/L of oxygen), near instantaneous response time and a flexible architecture for addition of multiple sensors.

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High fidelity fibre-based physiological sensing deep in tissue

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Michael George Tanner

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High fidelity fibre-based physiological sensing deep in tissue

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Michael George Tanner

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