High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Rory Duncan; Andrew C. Don-Wauchope; Sompol Tapechum; Michael J. Shipston; Robert H. Chow; Peter Estibeiro

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Rory Duncan, Andrew C. Don-Wauchope, Sompol Tapechum, Michael J. Shipston, Robert H. Chow, Peter Estibeiro

Research output: Contribution to journal › Article › peer-review

Abstract

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

Original language	English
Pages (from-to)	497-501
Number of pages	5
Journal	Biochemical Journal
Volume	342
Issue number	3
Publication status	Published - 15 Sept 1999

Keywords

Adrenal Medulla
Animals
Cattle
Chromaffin Cells
Culture Media
Electrophysiology
Exocytosis
Gene Transfer Techniques
Green Fluorescent Proteins
Luminescent Proteins
Mice
Microscopy, Confocal
Polymerase Chain Reaction
Semliki forest virus

Cite this

@article{45095be0df914decbc5c46a3f863f19b,

title = "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells",

abstract = "Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.",

keywords = "Adrenal Medulla, Animals, Cattle, Chromaffin Cells, Culture Media, Electrophysiology, Exocytosis, Gene Transfer Techniques, Green Fluorescent Proteins, Luminescent Proteins, Mice, Microscopy, Confocal, Polymerase Chain Reaction, Semliki forest virus",

author = "Rory Duncan and Don-Wauchope, \{Andrew C.\} and Sompol Tapechum and Shipston, \{Michael J.\} and Chow, \{Robert H.\} and Peter Estibeiro",

year = "1999",

month = sep,

day = "15",

language = "English",

volume = "342",

pages = "497--501",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

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T1 - High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

AU - Duncan, Rory

AU - Don-Wauchope, Andrew C.

AU - Tapechum, Sompol

AU - Shipston, Michael J.

AU - Chow, Robert H.

AU - Estibeiro, Peter

PY - 1999/9/15

Y1 - 1999/9/15

N2 - Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

AB - Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

KW - Adrenal Medulla

KW - Animals

KW - Cattle

KW - Chromaffin Cells

KW - Culture Media

KW - Electrophysiology

KW - Exocytosis

KW - Gene Transfer Techniques

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Mice

KW - Microscopy, Confocal

KW - Polymerase Chain Reaction

KW - Semliki forest virus

UR - https://www.scopus.com/pages/publications/0033568391

M3 - Article

C2 - 10477258

SN - 0264-6021

VL - 342

SP - 497

EP - 501

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Abstract

Keywords

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