High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Rory Duncan, Andrew C. Don-Wauchope, Sompol Tapechum, Michael J. Shipston, Robert H. Chow, Peter Estibeiro

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalBiochemical Journal
Volume342
Issue number3
Publication statusPublished - 15 Sep 1999

Keywords

  • Adrenal Medulla
  • Animals
  • Cattle
  • Chromaffin Cells
  • Culture Media
  • Electrophysiology
  • Exocytosis
  • Gene Transfer Techniques
  • Green Fluorescent Proteins
  • Luminescent Proteins
  • Mice
  • Microscopy, Confocal
  • Polymerase Chain Reaction
  • Semliki forest virus

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  • Cite this

    Duncan, R., Don-Wauchope, A. C., Tapechum, S., Shipston, M. J., Chow, R. H., & Estibeiro, P. (1999). High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells. Biochemical Journal, 342(3), 497-501.