High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Rory Duncan; Andrew C. Don-Wauchope; Sompol Tapechum; Michael J. Shipston; Robert H. Chow; Peter Estibeiro

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Rory Duncan, Andrew C. Don-Wauchope, Sompol Tapechum, Michael J. Shipston, Robert H. Chow, Peter Estibeiro

Research output: Contribution to journal › Article › peer-review

Abstract

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

Original language	English
Pages (from-to)	497-501
Number of pages	5
Journal	Biochemical Journal
Volume	342
Issue number	3
Publication status	Published - 15 Sept 1999

Keywords

Adrenal Medulla
Animals
Cattle
Chromaffin Cells
Culture Media
Electrophysiology
Exocytosis
Gene Transfer Techniques
Green Fluorescent Proteins
Luminescent Proteins
Mice
Microscopy, Confocal
Polymerase Chain Reaction
Semliki forest virus

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@article{45095be0df914decbc5c46a3f863f19b,

title = "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells",

abstract = "Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.",

keywords = "Adrenal Medulla, Animals, Cattle, Chromaffin Cells, Culture Media, Electrophysiology, Exocytosis, Gene Transfer Techniques, Green Fluorescent Proteins, Luminescent Proteins, Mice, Microscopy, Confocal, Polymerase Chain Reaction, Semliki forest virus",

author = "Rory Duncan and Don-Wauchope, {Andrew C.} and Sompol Tapechum and Shipston, {Michael J.} and Chow, {Robert H.} and Peter Estibeiro",

year = "1999",

month = sep,

day = "15",

language = "English",

volume = "342",

pages = "497--501",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

number = "3",

}

TY - JOUR

T1 - High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

AU - Duncan, Rory

AU - Don-Wauchope, Andrew C.

AU - Tapechum, Sompol

AU - Shipston, Michael J.

AU - Chow, Robert H.

AU - Estibeiro, Peter

PY - 1999/9/15

Y1 - 1999/9/15

N2 - Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

AB - Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

KW - Adrenal Medulla

KW - Animals

KW - Cattle

KW - Chromaffin Cells

KW - Culture Media

KW - Electrophysiology

KW - Exocytosis

KW - Gene Transfer Techniques

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Mice

KW - Microscopy, Confocal

KW - Polymerase Chain Reaction

KW - Semliki forest virus

M3 - Article

C2 - 10477258

SN - 0264-6021

VL - 342

SP - 497

EP - 501

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Abstract

Keywords

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