Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.

Mark Hartl, M Kilemade, D Sheehan, C Mothersill, J O'Halloran, N M O'Brien, F N A M van Pelt

    Research output: Contribution to journalArticle

    Abstract

    Hatchery-reared turbot (Scophthalmus maximus L.) were exposed for 3 weeks, under laboratory conditions, to sediment collected from polluted sites in Cork Harbour and a reference site at Ballymacoda, Co. Cork, Ireland. The potential of surficial sediment for inducing hepatic biomarkers was assessed at two levels of biological organisation: expression of cytochrome P450 Western blotting analysis and 7-ethoxy-resorufin O-dealkylase (EROD), 7-benzoxy resorufin O-dealkylase (BROD), 7-methoxy resorufin O-dealkylase (MROD), 7-pentoxy-resorufin O-dealkylase (PROD) activities and DNA integrity (Comet assay). Positive controls were generated, either by exposing turbot to cadmium chloride-spiked seawater (Comet assay) or to beta-naphthaflavone by intraperitoneal injection (cytochrome P450 induction). The induction of cytochrome P450 activity (EROD, MROD and PROD) in animals following a 7-day exposure to contaminated sediments was significantly higher than those exposed to reference site sediment and remained elevated thereafter; BROD was not induced. DNA single-strand breaks were also significantly higher following exposure to contaminated sediments throughout the experiment. Although no direct correlation between induction of alkoxyresorufin O-dealkylase activities and a particular chemical class was established, the induction of MROD and PROD activities in fish exposed to sediments containing complex contaminant mixtures, appeared to be more sensitive than conventional EROD activity assays. We conclude from the present laboratory study that S. maximus is a suitable sentinel species for the assessment of moderately contaminated sediments and therefore allows for the further development of this model for future, ecologically relevant, field studies.

    Original languageEnglish
    Pages (from-to)191-208
    Number of pages18
    JournalMarine Environmental Research
    Volume64
    Issue number2
    DOIs
    Publication statusPublished - Aug 2007

    Fingerprint

    Flatfishes
    Biomarkers
    Liver
    Cytochrome P-450 Enzyme System
    Comet Assay
    resorufin
    Single-Stranded DNA Breaks
    Cadmium Chloride
    Seawater
    Intraperitoneal Injections
    Complex Mixtures
    Ireland
    Fishes

    Keywords

    • Sediment
    • Cytochrome P450
    • Western blotting
    • Comet assay
    • Pleuronectiformes

    Cite this

    Hartl, M., Kilemade, M., Sheehan, D., Mothersill, C., O'Halloran, J., O'Brien, N. M., & Pelt, F. N. A. M. V. (2007). Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L. Marine Environmental Research, 64(2), 191-208. https://doi.org/10.1016/j.marenvres.2007.01.002
    Hartl, Mark ; Kilemade, M ; Sheehan, D ; Mothersill, C ; O'Halloran, J ; O'Brien, N M ; Pelt, F N A M van. / Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L. In: Marine Environmental Research. 2007 ; Vol. 64, No. 2. pp. 191-208.
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    Hartl, M, Kilemade, M, Sheehan, D, Mothersill, C, O'Halloran, J, O'Brien, NM & Pelt, FNAMV 2007, 'Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.', Marine Environmental Research, vol. 64, no. 2, pp. 191-208. https://doi.org/10.1016/j.marenvres.2007.01.002

    Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L. / Hartl, Mark; Kilemade, M; Sheehan, D; Mothersill, C; O'Halloran, J; O'Brien, N M; Pelt, F N A M van.

    In: Marine Environmental Research, Vol. 64, No. 2, 08.2007, p. 191-208.

    Research output: Contribution to journalArticle

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    T1 - Hepatic biomarkers of sediment-associated pollution in juvenile turbot, Scophthalmus maximus L.

    AU - Hartl, Mark

    AU - Kilemade, M

    AU - Sheehan, D

    AU - Mothersill, C

    AU - O'Halloran, J

    AU - O'Brien, N M

    AU - Pelt, F N A M van

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    KW - Western blotting

    KW - Comet assay

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