Heat in optical tweezers

B. Del Rosal; P. Haro-González; W. T. Ramsay; L. M. Maestro; K. Santacruz-Gómez; M. C. Iglesias-De La Cruz; F. Sanz-Rodríguez; J. Y. Chooi; P. Rodríguez-Sevilla; D. Choudhury; A. K. Kar; J. G. García-Solé; L. Patterson; D. Jaque

doi:10.1117/12.2027750

Heat in optical tweezers

B. Del Rosal, P. Haro-González, W. T. Ramsay, L. M. Maestro, K. Santacruz-Gómez, M. C. Iglesias-De La Cruz, F. Sanz-Rodríguez, J. Y. Chooi, P. Rodríguez-Sevilla, D. Choudhury, A. K. Kar, J. G. García-Solé, L. Patterson, D. Jaque^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Citations (Scopus)

Abstract

Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Volume	8810
DOIs	https://doi.org/10.1117/12.2027750
Publication status	Published - 11 Dec 2013
Event	Optical Trapping and Optical Micromanipulation X - San Diego, CA, United States Duration: 25 Aug 2013 → 29 Aug 2013

Conference

Conference	Optical Trapping and Optical Micromanipulation X
Country/Territory	United States
City	San Diego, CA
Period	25/08/13 → 29/08/13

Keywords

fluorescence imaging
lymphocytes
microspheres
nanothermometry
Optical trapping
quantum dots

Access to Document

10.1117/12.2027750

Cite this

Del Rosal, B., Haro-González, P., Ramsay, W. T., Maestro, L. M., Santacruz-Gómez, K., Iglesias-De La Cruz, M. C., Sanz-Rodríguez, F., Chooi, J. Y., Rodríguez-Sevilla, P., Choudhury, D., Kar, A. K., García-Solé, J. G., Patterson, L., & Jaque, D. (2013). Heat in optical tweezers. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8810). Article 88102A https://doi.org/10.1117/12.2027750

@inproceedings{630a29c6d97748868ff0e31fae790cf4,

title = "Heat in optical tweezers",

abstract = "Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.",

keywords = "fluorescence imaging, lymphocytes, microspheres, nanothermometry, Optical trapping, quantum dots",

author = "{Del Rosal}, B. and P. Haro-Gonz{\'a}lez and Ramsay, {W. T.} and Maestro, {L. M.} and K. Santacruz-G{\'o}mez and {Iglesias-De La Cruz}, {M. C.} and F. Sanz-Rodr{\'i}guez and Chooi, {J. Y.} and P. Rodr{\'i}guez-Sevilla and D. Choudhury and Kar, {A. K.} and Garc{\'i}a-Sol{\'e}, {J. G.} and L. Patterson and D. Jaque",

year = "2013",

month = dec,

day = "11",

doi = "10.1117/12.2027750",

language = "English",

isbn = "9780819496607",

volume = "8810",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "Optical Trapping and Optical Micromanipulation X ; Conference date: 25-08-2013 Through 29-08-2013",

}

Del Rosal, B, Haro-González, P, Ramsay, WT, Maestro, LM, Santacruz-Gómez, K, Iglesias-De La Cruz, MC, Sanz-Rodríguez, F, Chooi, JY, Rodríguez-Sevilla, P, Choudhury, D, Kar, AK, García-Solé, JG, Patterson, L & Jaque, D 2013, Heat in optical tweezers. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 8810, 88102A, Optical Trapping and Optical Micromanipulation X, San Diego, CA, United States, 25/08/13. https://doi.org/10.1117/12.2027750

TY - GEN

T1 - Heat in optical tweezers

AU - Del Rosal, B.

AU - Haro-González, P.

AU - Ramsay, W. T.

AU - Maestro, L. M.

AU - Santacruz-Gómez, K.

AU - Iglesias-De La Cruz, M. C.

AU - Sanz-Rodríguez, F.

AU - Chooi, J. Y.

AU - Rodríguez-Sevilla, P.

AU - Choudhury, D.

AU - Kar, A. K.

AU - García-Solé, J. G.

AU - Patterson, L.

AU - Jaque, D.

PY - 2013/12/11

Y1 - 2013/12/11

N2 - Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

AB - Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

KW - fluorescence imaging

KW - lymphocytes

KW - microspheres

KW - nanothermometry

KW - Optical trapping

KW - quantum dots

U2 - 10.1117/12.2027750

DO - 10.1117/12.2027750

M3 - Conference contribution

AN - SCOPUS:84889570954

SN - 9780819496607

VL - 8810

BT - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Optical Trapping and Optical Micromanipulation X

Y2 - 25 August 2013 through 29 August 2013

ER -

Heat in optical tweezers

Abstract

Conference

Keywords

Access to Document

Fingerprint

Cite this