Heat in optical tweezers

B. Del Rosal, P. Haro-González, W. T. Ramsay, L. M. Maestro, K. Santacruz-Gómez, M. C. Iglesias-De La Cruz, F. Sanz-Rodríguez, J. Y. Chooi, P. Rodríguez-Sevilla, D. Choudhury, A. K. Kar, J. G. García-Solé, L. Patterson, D. Jaque

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Publication statusPublished - 11 Dec 2013
EventOptical Trapping and Optical Micromanipulation X - San Diego, CA, United States
Duration: 25 Aug 201329 Aug 2013


ConferenceOptical Trapping and Optical Micromanipulation X
CountryUnited States
CitySan Diego, CA


  • fluorescence imaging
  • lymphocytes
  • microspheres
  • nanothermometry
  • Optical trapping
  • quantum dots

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    Del Rosal, B., Haro-González, P., Ramsay, W. T., Maestro, L. M., Santacruz-Gómez, K., Iglesias-De La Cruz, M. C., Sanz-Rodríguez, F., Chooi, J. Y., Rodríguez-Sevilla, P., Choudhury, D., Kar, A. K., García-Solé, J. G., Patterson, L., & Jaque, D. (2013). Heat in optical tweezers. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8810). [88102A] https://doi.org/10.1117/12.2027750