Gram-negative bacteria associated with brewery yeasts

Reclassification of Obesumbacterium proteus biogroup 2 as Shimwellia pseudoproteus gen. nov., sp. nov., and transfer of Escherichia blattae to Shimwellia blattae comb. nov.

Fergus G. Priest, Margaret Barker

    Research output: Contribution to journalArticle

    Abstract

    Phylogenetic analyses of type and reference strains of Obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16S rRNA gene sequences and partial sequences of four protein-coding genes (fusA, leuS, pyrG and rpoB). Both approaches revealed that O. proteus biogroup 1 strains were closely related to Hafnia alvei. Biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included Escherichia blattae. Phenotypic tests were consistent with the molecular classification and provided diagnostic features. It is proposed that biogroup 2 strains be placed in a new genus, Shimwellia gen. nov., as Shimwellia pseudoproteus sp. nov., with strain 521T (5DSM 3038T5LMG 24835T5NCIMB 14534T) as the type strain, and that Escherichia blattae be transferred to the genus Shimwellia as Shimwellia blattae comb. nov., with strain ATCC 29907T (=DSM 4481T) as the type strain. © 2010 IUMS.

    Original languageEnglish
    Pages (from-to)828-833
    Number of pages6
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume60
    Issue number4
    DOIs
    Publication statusPublished - Apr 2010

    Fingerprint

    Escherichia
    Comb and Wattles
    Proteus
    Gram-Negative Bacteria
    Hafnia alvei
    Yeasts
    rRNA Genes
    Proteins

    Cite this

    @article{b11f7c99d1ef4d9d9e09d96e46145cbe,
    title = "Gram-negative bacteria associated with brewery yeasts: Reclassification of Obesumbacterium proteus biogroup 2 as Shimwellia pseudoproteus gen. nov., sp. nov., and transfer of Escherichia blattae to Shimwellia blattae comb. nov.",
    abstract = "Phylogenetic analyses of type and reference strains of Obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16S rRNA gene sequences and partial sequences of four protein-coding genes (fusA, leuS, pyrG and rpoB). Both approaches revealed that O. proteus biogroup 1 strains were closely related to Hafnia alvei. Biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included Escherichia blattae. Phenotypic tests were consistent with the molecular classification and provided diagnostic features. It is proposed that biogroup 2 strains be placed in a new genus, Shimwellia gen. nov., as Shimwellia pseudoproteus sp. nov., with strain 521T (5DSM 3038T5LMG 24835T5NCIMB 14534T) as the type strain, and that Escherichia blattae be transferred to the genus Shimwellia as Shimwellia blattae comb. nov., with strain ATCC 29907T (=DSM 4481T) as the type strain. {\circledC} 2010 IUMS.",
    author = "Priest, {Fergus G.} and Margaret Barker",
    year = "2010",
    month = "4",
    doi = "10.1099/ijs.0.013458-0",
    language = "English",
    volume = "60",
    pages = "828--833",
    journal = "International Journal of Systematic and Evolutionary Microbiology",
    issn = "1466-5026",
    publisher = "Society for General Microbiology",
    number = "4",

    }

    TY - JOUR

    T1 - Gram-negative bacteria associated with brewery yeasts

    T2 - Reclassification of Obesumbacterium proteus biogroup 2 as Shimwellia pseudoproteus gen. nov., sp. nov., and transfer of Escherichia blattae to Shimwellia blattae comb. nov.

    AU - Priest, Fergus G.

    AU - Barker, Margaret

    PY - 2010/4

    Y1 - 2010/4

    N2 - Phylogenetic analyses of type and reference strains of Obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16S rRNA gene sequences and partial sequences of four protein-coding genes (fusA, leuS, pyrG and rpoB). Both approaches revealed that O. proteus biogroup 1 strains were closely related to Hafnia alvei. Biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included Escherichia blattae. Phenotypic tests were consistent with the molecular classification and provided diagnostic features. It is proposed that biogroup 2 strains be placed in a new genus, Shimwellia gen. nov., as Shimwellia pseudoproteus sp. nov., with strain 521T (5DSM 3038T5LMG 24835T5NCIMB 14534T) as the type strain, and that Escherichia blattae be transferred to the genus Shimwellia as Shimwellia blattae comb. nov., with strain ATCC 29907T (=DSM 4481T) as the type strain. © 2010 IUMS.

    AB - Phylogenetic analyses of type and reference strains of Obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16S rRNA gene sequences and partial sequences of four protein-coding genes (fusA, leuS, pyrG and rpoB). Both approaches revealed that O. proteus biogroup 1 strains were closely related to Hafnia alvei. Biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included Escherichia blattae. Phenotypic tests were consistent with the molecular classification and provided diagnostic features. It is proposed that biogroup 2 strains be placed in a new genus, Shimwellia gen. nov., as Shimwellia pseudoproteus sp. nov., with strain 521T (5DSM 3038T5LMG 24835T5NCIMB 14534T) as the type strain, and that Escherichia blattae be transferred to the genus Shimwellia as Shimwellia blattae comb. nov., with strain ATCC 29907T (=DSM 4481T) as the type strain. © 2010 IUMS.

    UR - http://www.scopus.com/inward/record.url?scp=77950618280&partnerID=8YFLogxK

    U2 - 10.1099/ijs.0.013458-0

    DO - 10.1099/ijs.0.013458-0

    M3 - Article

    VL - 60

    SP - 828

    EP - 833

    JO - International Journal of Systematic and Evolutionary Microbiology

    JF - International Journal of Systematic and Evolutionary Microbiology

    SN - 1466-5026

    IS - 4

    ER -