Gill protein turnover: Costs of adaptation

A. R. Lyndon, D. F. Houlihan

    Research output: Contribution to journalArticle

    Abstract

    Measurements of gill protein synthesis, and hence turnover, were greatly facilitated over the last decade by the application of 'flooding dose' methodology to non-mammalian species. Numerous studies show that in fish and aquatic invertebrates, gills are among the most active tissues with respect to protein turnover, this being true under a variety of environmental and nutritional conditions. The main components being turned over in fish gills are probably collagen, primarily in the gill arches, and epithelial cell proteins in the filaments, both arches and filaments having similar protein synthesis rates. Intriguingly, differences are apparent between protein synthesis rates of adjacent holobranchs, the first (most anterior) being significantly more active than the second or third, perhaps hinting at functional differences between holobranchs. Experimental estimates of energetic costs for protein synthesis, derived from cycloheximide treatment of isolated perfused gills, give a maximum value of 14 mmol O2/g protein synthesized, which is about double theoretical costs. Environmental stressors, such as heavy metals or acid/aluminum, have variable effects on branchial protein turnover. Limited data suggest that zinc or acid exposure depresses protein synthesis, whereas acid/aluminum increases it quite markedly. Calculations indicate that whereas effects within the gilts may be substantial, in terms of whole animal energetics, the costs of branchial adaptation are likely to be small.

    Original languageEnglish
    Pages (from-to)27-34
    Number of pages8
    JournalComparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology
    Volume119
    Issue number1
    DOIs
    Publication statusPublished - Jan 1998

    Fingerprint

    protein metabolism
    gills
    protein synthesis
    aluminum
    acids
    aquatic invertebrates
    cycloheximide
    fish
    gilts
    collagen
    heavy metals
    epithelial cells
    proteins
    zinc
    dosage
    animals

    Keywords

    • Adaptation
    • Energetic costs
    • Environmental perturbation
    • Fish
    • Gills
    • Invertebrates
    • Protein synthesis
    • Turnover

    Cite this

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    Gill protein turnover : Costs of adaptation. / Lyndon, A. R.; Houlihan, D. F.

    In: Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, Vol. 119, No. 1, 01.1998, p. 27-34.

    Research output: Contribution to journalArticle

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