Genomic analysis of the phosphotransferase system in Clostridium botulinum

Wilfrid J. Mitchell, Parul Tewatia, Philip G. Meaden

Research output: Contribution to journalLiterature review

Abstract

Clostridium botulinum is capable of fermenting carbohydrates, but there have been no detailed studies of the uptake of sugars and related substrates. In bacteria, a common and often predominant system of carbohydrate uptake is the phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS). This multi-protein complex catalyses a group translocation involving both uptake and phosphorylation of carbohydrates, and is also known to play an important role in environmental sensing and metabolic regulation. The genome of C. botulinum encodes 15 PTSs which have a similar domain structure to the PTS in other bacteria. Based on phylogenetic relationships and analysis of gene clusters, the C. botulinum PTS appears to be involved in the uptake of hexoses, hexose derivatives and disaccharides. C. botulinum also contains the components of PTS-associated regulatory mechanisms which have been characterised in other bacteria. It therefore seems likely that the PTS plays a significant, and previously unrecognised, role in the physiology of this bacterium. Copyright © 2007 S. Karger AG.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalJournal of Molecular Microbiology and Biotechnology
Volume12
Issue number1-2
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Clostridium botulinum
Systems Analysis
Phosphotransferases
Bacteria
Hexoses
Carbohydrates
Phosphoenolpyruvate
Disaccharides
Multigene Family
Phosphorylation
Genome
Proteins

Keywords

  • Phosphotransferase system
  • Sugar uptake
  • Transport

Cite this

Mitchell, Wilfrid J. ; Tewatia, Parul ; Meaden, Philip G. / Genomic analysis of the phosphotransferase system in Clostridium botulinum. In: Journal of Molecular Microbiology and Biotechnology. 2006 ; Vol. 12, No. 1-2. pp. 33-42.
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Genomic analysis of the phosphotransferase system in Clostridium botulinum. / Mitchell, Wilfrid J.; Tewatia, Parul; Meaden, Philip G.

In: Journal of Molecular Microbiology and Biotechnology, Vol. 12, No. 1-2, 12.2006, p. 33-42.

Research output: Contribution to journalLiterature review

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AB - Clostridium botulinum is capable of fermenting carbohydrates, but there have been no detailed studies of the uptake of sugars and related substrates. In bacteria, a common and often predominant system of carbohydrate uptake is the phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS). This multi-protein complex catalyses a group translocation involving both uptake and phosphorylation of carbohydrates, and is also known to play an important role in environmental sensing and metabolic regulation. The genome of C. botulinum encodes 15 PTSs which have a similar domain structure to the PTS in other bacteria. Based on phylogenetic relationships and analysis of gene clusters, the C. botulinum PTS appears to be involved in the uptake of hexoses, hexose derivatives and disaccharides. C. botulinum also contains the components of PTS-associated regulatory mechanisms which have been characterised in other bacteria. It therefore seems likely that the PTS plays a significant, and previously unrecognised, role in the physiology of this bacterium. Copyright © 2007 S. Karger AG.

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