Genetic transfer of lactate-utilizing ability in the rumen bacterium Selenomonas ruminantium

M. Gilmour, W. J. Mitchell, H. J. Flint

Research output: Contribution to journalArticle

Abstract

Matings between the lactate-utilizing, tetracycline-sensitive Selenomonas ruminantium strains 5521C1 and 5934e and the lactate-non-utilizing, tetracycline-resistant strain FB322 resulted in putative recombinant strains capable of growth on lactate. Analysis of total protein by SDS-PAGE and chromosomal DNA by hybridization, indicated that the recombinants were derived from strain FB322. DNA hybridization produced no evidence that plasmid transfer occurred, leaving chromosomal DNA transfer as the most likely mechanism for the altered phenotype. Analysis of strains 5934e, FB322 and the resulting recombinant TC3 indicated that all three strains contained D-nLDH and L-nLDH activities. In addition strains 5934e and TC3 possessed D-iLDH activity when grown on DL-lactate. The ability of strain FB322 to grow on pyruvate but not lactate suggested that the lactate-utilizing recombinant had acquired the ability to synthesize D-iLDH.

Original languageEnglish
Pages (from-to)52-56
Number of pages5
JournalLetters in Applied Microbiology
Volume22
Issue number1
DOIs
Publication statusPublished - 1996

Fingerprint

Selenomonas
Rumen
Lactic Acid
Bacteria
Tetracycline
DNA
Pyruvic Acid
Polyacrylamide Gel Electrophoresis
Plasmids
Phenotype
Growth

Cite this

Gilmour, M. ; Mitchell, W. J. ; Flint, H. J. / Genetic transfer of lactate-utilizing ability in the rumen bacterium Selenomonas ruminantium. In: Letters in Applied Microbiology. 1996 ; Vol. 22, No. 1. pp. 52-56.
@article{555e706dd6f045fab7f222672b162f07,
title = "Genetic transfer of lactate-utilizing ability in the rumen bacterium Selenomonas ruminantium",
abstract = "Matings between the lactate-utilizing, tetracycline-sensitive Selenomonas ruminantium strains 5521C1 and 5934e and the lactate-non-utilizing, tetracycline-resistant strain FB322 resulted in putative recombinant strains capable of growth on lactate. Analysis of total protein by SDS-PAGE and chromosomal DNA by hybridization, indicated that the recombinants were derived from strain FB322. DNA hybridization produced no evidence that plasmid transfer occurred, leaving chromosomal DNA transfer as the most likely mechanism for the altered phenotype. Analysis of strains 5934e, FB322 and the resulting recombinant TC3 indicated that all three strains contained D-nLDH and L-nLDH activities. In addition strains 5934e and TC3 possessed D-iLDH activity when grown on DL-lactate. The ability of strain FB322 to grow on pyruvate but not lactate suggested that the lactate-utilizing recombinant had acquired the ability to synthesize D-iLDH.",
author = "M. Gilmour and Mitchell, {W. J.} and Flint, {H. J.}",
year = "1996",
doi = "10.1111/j.1472-765X.1996.tb01107.x",
language = "English",
volume = "22",
pages = "52--56",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "1",

}

Genetic transfer of lactate-utilizing ability in the rumen bacterium Selenomonas ruminantium. / Gilmour, M.; Mitchell, W. J.; Flint, H. J.

In: Letters in Applied Microbiology, Vol. 22, No. 1, 1996, p. 52-56.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic transfer of lactate-utilizing ability in the rumen bacterium Selenomonas ruminantium

AU - Gilmour, M.

AU - Mitchell, W. J.

AU - Flint, H. J.

PY - 1996

Y1 - 1996

N2 - Matings between the lactate-utilizing, tetracycline-sensitive Selenomonas ruminantium strains 5521C1 and 5934e and the lactate-non-utilizing, tetracycline-resistant strain FB322 resulted in putative recombinant strains capable of growth on lactate. Analysis of total protein by SDS-PAGE and chromosomal DNA by hybridization, indicated that the recombinants were derived from strain FB322. DNA hybridization produced no evidence that plasmid transfer occurred, leaving chromosomal DNA transfer as the most likely mechanism for the altered phenotype. Analysis of strains 5934e, FB322 and the resulting recombinant TC3 indicated that all three strains contained D-nLDH and L-nLDH activities. In addition strains 5934e and TC3 possessed D-iLDH activity when grown on DL-lactate. The ability of strain FB322 to grow on pyruvate but not lactate suggested that the lactate-utilizing recombinant had acquired the ability to synthesize D-iLDH.

AB - Matings between the lactate-utilizing, tetracycline-sensitive Selenomonas ruminantium strains 5521C1 and 5934e and the lactate-non-utilizing, tetracycline-resistant strain FB322 resulted in putative recombinant strains capable of growth on lactate. Analysis of total protein by SDS-PAGE and chromosomal DNA by hybridization, indicated that the recombinants were derived from strain FB322. DNA hybridization produced no evidence that plasmid transfer occurred, leaving chromosomal DNA transfer as the most likely mechanism for the altered phenotype. Analysis of strains 5934e, FB322 and the resulting recombinant TC3 indicated that all three strains contained D-nLDH and L-nLDH activities. In addition strains 5934e and TC3 possessed D-iLDH activity when grown on DL-lactate. The ability of strain FB322 to grow on pyruvate but not lactate suggested that the lactate-utilizing recombinant had acquired the ability to synthesize D-iLDH.

UR - http://www.scopus.com/inward/record.url?scp=0030027304&partnerID=8YFLogxK

U2 - 10.1111/j.1472-765X.1996.tb01107.x

DO - 10.1111/j.1472-765X.1996.tb01107.x

M3 - Article

VL - 22

SP - 52

EP - 56

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 1

ER -