Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers

C. Tomaszewski, S.L. Byrne, A. Foito, S. Kildea, D. Kopeckì, J. Dolezel, J.S. Heslop-Harrison, D. Stewart, S. Barth

    Research output: Contribution to journalArticle

    Abstract

    Perennial ryegrass is the principal forage grass species used in temperate agriculture. In recent years, significant efforts have been made to develop molecular marker strategies to allow cost-effective characterization of a large number of loci simultaneously. One such strategy involves using DArT markers, and a DArT array has recently been developed for the Lolium-Festuca complex. In this study, we report the first use of the DArTFest array to generate a genetic linkage map based on 326 markers in a Lolium perenne F2 population, consisting of 325 genotypes. For proof of concept, the map was used to identify QTL associated with differences in crown rust susceptibility, caused by the fungal biotroph, Puccinia coronata.
    © 2012 Blackwell Verlag GmbH
    Original languageEnglish
    Pages (from-to)345-349
    JournalPlant Breeding
    Volume131
    Issue number2
    Early online date21 Feb 2012
    DOIs
    Publication statusPublished - Apr 2012

    Keywords

    • Lolium perenne
    • perennial ryegrass
    • genetic map
    • crown rust
    • Puccinia coronata
    • DArT

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  • Cite this

    Tomaszewski, C., Byrne, S. L., Foito, A., Kildea, S., Kopeckì, D., Dolezel, J., Heslop-Harrison, J. S., Stewart, D., & Barth, S. (2012). Genetic linkage mapping in an F2 perennial ryegrass population using DArT markers. Plant Breeding, 131(2), 345-349. https://doi.org/10.1111/j.1439-0523.2011.01944.x