Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs

Wouter Schuurman; Peter A. Levett; Michiel W. Pot; Paul Rene van Weeren; Wouter J. A. Dhert; Dietmar W. Hutmacher; Ferry P. W. Melchels; Travis J. Klein; Jos Malda

doi:10.1002/mabi.201200471

Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs

Wouter Schuurman, Peter A. Levett, Michiel W. Pot, Paul Rene van Weeren, Wouter J. A. Dhert, Dietmar W. Hutmacher, Ferry P. W. Melchels, Travis J. Klein, Jos Malda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

645 Citations (Scopus)

Abstract

Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 degrees C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

Original language	English
Pages (from-to)	551-561
Number of pages	11
Journal	Macromolecular Bioscience
Volume	13
Issue number	5
DOIs	https://doi.org/10.1002/mabi.201200471
Publication status	Published - May 2013

Keywords

additive manufacturing
cartilage
gelatin
hydrogels
tissue engineering
POLY(ETHYLENE GLYCOL) HYDROGELS
HYALURONIC-ACID HYDROGELS
ARTICULAR CHONDROCYTES
MECHANICAL-PROPERTIES
ECM PRODUCTION
CROSS-LINKING
STEM-CELLS
I COLLAGEN
THICKNESS
DIFFERENTIATION

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@article{d363dd84d4604690967a73fae87bc292,

title = "Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs",

abstract = "Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 degrees C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.",

keywords = "additive manufacturing, cartilage, gelatin, hydrogels, tissue engineering, POLY(ETHYLENE GLYCOL) HYDROGELS, HYALURONIC-ACID HYDROGELS, ARTICULAR CHONDROCYTES, MECHANICAL-PROPERTIES, ECM PRODUCTION, CROSS-LINKING, STEM-CELLS, I COLLAGEN, THICKNESS, DIFFERENTIATION",

author = "Wouter Schuurman and Levett, {Peter A.} and Pot, {Michiel W.} and {van Weeren}, {Paul Rene} and Dhert, {Wouter J. A.} and Hutmacher, {Dietmar W.} and Melchels, {Ferry P. W.} and Klein, {Travis J.} and Jos Malda",

year = "2013",

month = may,

doi = "10.1002/mabi.201200471",

language = "English",

volume = "13",

pages = "551--561",

journal = "Macromolecular Bioscience",

issn = "1616-5187",

publisher = "Wiley-VCH Verlag",

number = "5",

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TY - JOUR

T1 - Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs

AU - Schuurman, Wouter

AU - Levett, Peter A.

AU - Pot, Michiel W.

AU - van Weeren, Paul Rene

AU - Dhert, Wouter J. A.

AU - Hutmacher, Dietmar W.

AU - Melchels, Ferry P. W.

AU - Klein, Travis J.

AU - Malda, Jos

PY - 2013/5

Y1 - 2013/5

N2 - Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 degrees C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

AB - Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 degrees C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

KW - additive manufacturing

KW - cartilage

KW - gelatin

KW - hydrogels

KW - tissue engineering

KW - POLY(ETHYLENE GLYCOL) HYDROGELS

KW - HYALURONIC-ACID HYDROGELS

KW - ARTICULAR CHONDROCYTES

KW - MECHANICAL-PROPERTIES

KW - ECM PRODUCTION

KW - CROSS-LINKING

KW - STEM-CELLS

KW - I COLLAGEN

KW - THICKNESS

KW - DIFFERENTIATION

U2 - 10.1002/mabi.201200471

DO - 10.1002/mabi.201200471

M3 - Article

C2 - 23420700

SN - 1616-5187

VL - 13

SP - 551

EP - 561

JO - Macromolecular Bioscience

JF - Macromolecular Bioscience

IS - 5

ER -

Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs

Abstract

Keywords

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