Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs

Wouter Schuurman, Peter A. Levett, Michiel W. Pot, Paul Rene van Weeren, Wouter J. A. Dhert, Dietmar W. Hutmacher, Ferry P. W. Melchels, Travis J. Klein, Jos Malda

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Abstract

Gelatin-methacrylamide (gelMA) hydrogels are shown to support chondrocyte viability and differentiation and give wide ranging mechanical properties depending on several cross-linking parameters. Polymer concentration, UV exposure time, and thermal gelation prior to UV exposure allow for control over hydrogel stiffness and swelling properties. GelMA solutions have a low viscosity at 37 degrees C, which is incompatible with most biofabrication approaches. However, incorporation of hyaluronic acid (HA) and/or co-deposition with thermoplastics allows gelMA to be used in biofabrication processes. These attributes may allow engineered constructs to match the natural functional variations in cartilage mechanical and geometrical properties.

Original languageEnglish
Pages (from-to)551-561
Number of pages11
JournalMacromolecular Bioscience
Volume13
Issue number5
DOIs
Publication statusPublished - May 2013

Keywords

  • additive manufacturing
  • cartilage
  • gelatin
  • hydrogels
  • tissue engineering
  • POLY(ETHYLENE GLYCOL) HYDROGELS
  • HYALURONIC-ACID HYDROGELS
  • ARTICULAR CHONDROCYTES
  • MECHANICAL-PROPERTIES
  • ECM PRODUCTION
  • CROSS-LINKING
  • STEM-CELLS
  • I COLLAGEN
  • THICKNESS
  • DIFFERENTIATION

Cite this

Schuurman, W., Levett, P. A., Pot, M. W., van Weeren, P. R., Dhert, W. J. A., Hutmacher, D. W., Melchels, F. P. W., Klein, T. J., & Malda, J. (2013). Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs. Macromolecular Bioscience, 13(5), 551-561. https://doi.org/10.1002/mabi.201200471