Further progress towards a catalogue of all Arabidopsis genes: Analysis of a set of 5000 non-redundant ESTs

R Cooke, M Raynal, M Laudie, F Grellet, M Delseny, PC Morris, D Guerrier, J Giraudat, F Quigley, G Clabault, YF Li, R Mache, M Krivitzky, IJJ Gy, M Kreis, A Lecharny, Y Parmentier, J Marbach, J Fleck, B ClementG Philipps, C Herve, C Bardet, D Tremousaygue, B Lescure, C Lacomme, D Roby, MF Jourjon, P Chabrier, JL Charpenteau, T Desprez, J Amselem, H Chiapello, H Hofte

    Research output: Contribution to journalArticle

    174 Citations (Scopus)

    Abstract

    Nearly 7000 Arabidopsis thaliana-expressed sequence tags (ESTs) from 10 cDNA libraries have been sequenced, of which almost 5000 non-redundant tags have been submitted to the EMBL data bank. The quality of the cDNA libraries used is analysed. Similarity searches in international protein data banks have allowed the detection of significant similarities to a wide range of proteins from many organisms. Alignment with ESTs from the rice systematic sequencing project has allowed the detection of amino acid motifs which are conserved between the two organisms, thus identifying tags to genes encoding highly conserved proteins. These genes are candidates for a common framework in genome mapping projects in different plants.

    Original languageEnglish
    Pages (from-to)101-124
    Number of pages24
    JournalThe Plant Journal
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - Jan 1996

    Keywords

    • GENOME
    • HUMAN BRAIN
    • THALIANA
    • DATABASE
    • REVEALS
    • EXPRESSED SEQUENCE TAGS
    • RFLP

    Cite this