Functional, quantitative, and super-resolution imaging and spectroscopic approaches for studying exocytosis

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Regulated exocytosis is the process of secretion in specialized cells. Decades of intensive research have defined the molecules that drive the process of membrane fusion, as well as a plethora of accessory factors that shape and modulate the exocytotic function. As regulated exocytosis is generally a rapid event with a millisecond timescale, involving organelles on the nanometer scale, the field has long employed high spatial and temporal resolution techniques, including electrophysiology and, importantly, imaging approaches. Huge gains in the spatial and temporal resolution in biological microscopy have been delivered by recent hardware, engineering, and software breakthroughs; it is now possible to visualize and quantity the distributions, movements, and interactions of large cohorts of single protein molecules inside living cells. These technically demanding approaches have the potential to test long-standing hypotheses in the fields of membrane trafficking and exocytosis, building upon an extraordinary foundation of detailed biochemical and electrophysiological understanding.

Original languageEnglish
Title of host publicationExocytosis Methods
Pages75-91
Number of pages17
Volume83
ISBN (Electronic)978-1-62703-676-4
DOIs
Publication statusPublished - 2014

Publication series

NameNeuromethods
ISSN (Print)0893-2336

Keywords

  • FCS
  • FLIM
  • Fluorescence correlation spectroscopy
  • Fluorescence lifetime imaging microscopy
  • PALM
  • Photoactivation localization microscopy
  • Single-particle tracking
  • Stochastic optical reconstruction microscopy
  • STORM
  • Super-resolution
  • TIRFM
  • Total internal reflection fluorescence microscopy

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