Functional interplay between the Yersinia pseudotuberculosis YpsRI and YtbRI quorum sensing systems modulates swimming motility by controlling expression of flhDC and fliA

Steve Atkinson, Chien-Yi Chang, Hannah L. Patrick, Catherine M F Buckley, Yao Wang, R. Elizabeth Sockett, Miguel Cámara, Paul Williams

    Research output: Contribution to journalArticle

    Abstract

    Quorum sensing (QS) in Yersinia pseudotuberculosis involves two pairs of LuxRI orthologues (YpsRI and YtbRI) and multiple N-acylhomoserine lactones (AHLs). In a ypsI/ytbI mutant, AHL synthesis was abolished, unaffected in a ypsR/ytbR double mutant and substantially reduced in a ypsI/ytbR mutant, indicating that neither YpsR nor YtbR is essential for AHL synthesis. To determine the interrelationship between YpsRI and YtbRI we constructed chromosomal lux-promoter fusions to ypsR, ypsI, ytbR and ytbI and examined their expression in each of the QS mutant backgrounds. The YpsRI system negatively autoregulates itself but positively regulates the expression of the ytbRI system whereas the ytbRI system is positively autoregulated but only at the level of ytbI expression. YtbRI does not control expression of ypsR or ypsI. This hierarchical QS system controls swimming motility via regulation of flhDC and fliA. The AHLs synthesized via YtbI play a dual role, activating flhDC, in conjunction with YpsR but repressing fliA in conjunction with YtbR and YpsR. In liquid and plate assays, the early onset of motility observed in ypsR and ypsI mutants was abolished in ytbI, ytbR ypsI/ytbI, ypsR/ytbR mutants, indicating that QS regulates motility both positively (via YtbRI) and negatively (via YpsRI).

    Original languageEnglish
    Pages (from-to)137-151
    Number of pages15
    JournalMolecular Microbiology
    Volume69
    Issue number1
    DOIs
    Publication statusPublished - Jul 2008

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    Yersinia pseudotuberculosis
    Quorum Sensing
    Lactones

    ASJC Scopus subject areas

    • Molecular Biology
    • Microbiology

    Cite this

    Atkinson, Steve ; Chang, Chien-Yi ; Patrick, Hannah L. ; Buckley, Catherine M F ; Wang, Yao ; Sockett, R. Elizabeth ; Cámara, Miguel ; Williams, Paul. / Functional interplay between the Yersinia pseudotuberculosis YpsRI and YtbRI quorum sensing systems modulates swimming motility by controlling expression of flhDC and fliA. In: Molecular Microbiology. 2008 ; Vol. 69, No. 1. pp. 137-151.
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    abstract = "Quorum sensing (QS) in Yersinia pseudotuberculosis involves two pairs of LuxRI orthologues (YpsRI and YtbRI) and multiple N-acylhomoserine lactones (AHLs). In a ypsI/ytbI mutant, AHL synthesis was abolished, unaffected in a ypsR/ytbR double mutant and substantially reduced in a ypsI/ytbR mutant, indicating that neither YpsR nor YtbR is essential for AHL synthesis. To determine the interrelationship between YpsRI and YtbRI we constructed chromosomal lux-promoter fusions to ypsR, ypsI, ytbR and ytbI and examined their expression in each of the QS mutant backgrounds. The YpsRI system negatively autoregulates itself but positively regulates the expression of the ytbRI system whereas the ytbRI system is positively autoregulated but only at the level of ytbI expression. YtbRI does not control expression of ypsR or ypsI. This hierarchical QS system controls swimming motility via regulation of flhDC and fliA. The AHLs synthesized via YtbI play a dual role, activating flhDC, in conjunction with YpsR but repressing fliA in conjunction with YtbR and YpsR. In liquid and plate assays, the early onset of motility observed in ypsR and ypsI mutants was abolished in ytbI, ytbR ypsI/ytbI, ypsR/ytbR mutants, indicating that QS regulates motility both positively (via YtbRI) and negatively (via YpsRI).",
    author = "Steve Atkinson and Chien-Yi Chang and Patrick, {Hannah L.} and Buckley, {Catherine M F} and Yao Wang and Sockett, {R. Elizabeth} and Miguel C{\'a}mara and Paul Williams",
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    Functional interplay between the Yersinia pseudotuberculosis YpsRI and YtbRI quorum sensing systems modulates swimming motility by controlling expression of flhDC and fliA. / Atkinson, Steve; Chang, Chien-Yi; Patrick, Hannah L.; Buckley, Catherine M F; Wang, Yao; Sockett, R. Elizabeth; Cámara, Miguel; Williams, Paul.

    In: Molecular Microbiology, Vol. 69, No. 1, 07.2008, p. 137-151.

    Research output: Contribution to journalArticle

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    AU - Atkinson, Steve

    AU - Chang, Chien-Yi

    AU - Patrick, Hannah L.

    AU - Buckley, Catherine M F

    AU - Wang, Yao

    AU - Sockett, R. Elizabeth

    AU - Cámara, Miguel

    AU - Williams, Paul

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