'Finger print' analysis of cell wall proteins from different yeast species after trypsinization

Cell walls of 30 strains of yeasts, representing 7 genera were digested with trypsin and the resulting peptides in the supernatant fractions analysed by the 'finger print' technique. The distribution of ninhydrin positive components showed variations with different strains of yeasts. Twenty six of the 30 strains examined could be allocated to one or other of 4 'finger print' groups. Analysis of the peptides of Candida tropicalis (NCYC 405) cell walls after acid hydrolysis was also carried out to characterize the constituent amino acids. The adaptability of this technique to the study of cell wall proteins and its possible use in yeast taxonomy is discussed.