'Finger print' analysis of cell wall proteins from different yeast species after trypsinization

D J Stewart, S Widanapatirana

    Research output: Contribution to journalArticle

    Abstract

    Cell walls of 30 strains of yeasts, representing 7 genera were digested with trypsin and the resulting peptides in the supernatant fractions analysed by the 'finger print' technique. The distribution of ninhydrin positive components showed variations with different strains of yeasts. Twenty six of the 30 strains examined could be allocated to one or other of 4 'finger print' groups. Analysis of the peptides of Candida tropicalis (NCYC 405) cell walls after acid hydrolysis was also carried out to characterize the constituent amino acids. The adaptability of this technique to the study of cell wall proteins and its possible use in yeast taxonomy is discussed.

    Original languageEnglish
    Pages (from-to)167-172
    Number of pages6
    JournalMicrobios
    Volume9
    Issue number35
    Publication statusPublished - 1974

    Fingerprint

    cell walls
    yeasts
    peptides
    Candida tropicalis
    proteins
    acid hydrolysis
    trypsin
    taxonomy
    amino acids
    methodology

    Cite this

    Stewart, D J ; Widanapatirana, S. / 'Finger print' analysis of cell wall proteins from different yeast species after trypsinization. In: Microbios. 1974 ; Vol. 9, No. 35. pp. 167-172.
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    Stewart, DJ & Widanapatirana, S 1974, ''Finger print' analysis of cell wall proteins from different yeast species after trypsinization', Microbios, vol. 9, no. 35, pp. 167-172.

    'Finger print' analysis of cell wall proteins from different yeast species after trypsinization. / Stewart, D J; Widanapatirana, S.

    In: Microbios, Vol. 9, No. 35, 1974, p. 167-172.

    Research output: Contribution to journalArticle

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