Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays

Katjana Ehrlich, A. Kufcsák, N. Krstajić, R. K. Henderson, Robert R. Thomson, Michael George Tanner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In vivo fibre optic fluorescence-based sensing is the use of synthesised fluorophores which interrogate the local environment via variation in their fluorescence emission, addressed through an optic fibre. However, the emission intensity is influenced by intrinsic factors such as photobleaching, quantitative factors like concentration dependency and background signals from autofluorescence of tissue and the delivery optical fibre. Many of these problems can be addressed by using time-resolved spectroscopy which measures variations in the fluorescent lifetime. We present a versatile fibre-based time-resolved spectrograph based on a CMOS SPAD line sensor capable of acquiring time and spectral resolved fluorescent lifetime data in a single measurement exploiting the time-correlated single photon counting (TCSPC) technique. It is shown that these TCSPC histograms enable the differentiation between autofluorescence of tissue and synthesized fluorophores, as well as the removal of unwanted fibre background through post-processed time-gating. As a proof-of-principle application the pH- dependent changes in fluorescent lifetime of 5-carboxyuorescein (FAM) are measured.
LanguageEnglish
Title of host publicationOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII
EditorsIsrael Gannot
PublisherSPIE
Volume10058
ISBN (Print)9781510605572
DOIs
StatePublished - 28 Feb 2017

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume10058
ISSN (Print)0277-786X

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fiber optics
CMOS
spectroscopy
life (durability)
counting
fluorescence
fibers
photons
histograms
spectrographs
delivery
optical fibers
sensors

Cite this

Ehrlich, K., Kufcsák, A., Krstajić, N., Henderson, R. K., Thomson, R. R., & Tanner, M. G. (2017). Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays. In I. Gannot (Ed.), Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII (Vol. 10058). [100580H] (Proceedings of SPIE; Vol. 10058). SPIE. DOI: 10.1117/12.2252090
Ehrlich, Katjana ; Kufcsák, A. ; Krstajić, N. ; Henderson, R. K. ; Thomson, Robert R. ; Tanner, Michael George. / Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays. Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII. editor / Israel Gannot. Vol. 10058 SPIE, 2017. (Proceedings of SPIE).
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abstract = "In vivo fibre optic fluorescence-based sensing is the use of synthesised fluorophores which interrogate the local environment via variation in their fluorescence emission, addressed through an optic fibre. However, the emission intensity is influenced by intrinsic factors such as photobleaching, quantitative factors like concentration dependency and background signals from autofluorescence of tissue and the delivery optical fibre. Many of these problems can be addressed by using time-resolved spectroscopy which measures variations in the fluorescent lifetime. We present a versatile fibre-based time-resolved spectrograph based on a CMOS SPAD line sensor capable of acquiring time and spectral resolved fluorescent lifetime data in a single measurement exploiting the time-correlated single photon counting (TCSPC) technique. It is shown that these TCSPC histograms enable the differentiation between autofluorescence of tissue and synthesized fluorophores, as well as the removal of unwanted fibre background through post-processed time-gating. As a proof-of-principle application the pH- dependent changes in fluorescent lifetime of 5-carboxyuorescein (FAM) are measured.",
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Ehrlich, K, Kufcsák, A, Krstajić, N, Henderson, RK, Thomson, RR & Tanner, MG 2017, Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays. in I Gannot (ed.), Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII. vol. 10058, 100580H, Proceedings of SPIE, vol. 10058, SPIE. DOI: 10.1117/12.2252090

Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays. / Ehrlich, Katjana; Kufcsák, A.; Krstajić, N.; Henderson, R. K.; Thomson, Robert R.; Tanner, Michael George.

Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII. ed. / Israel Gannot. Vol. 10058 SPIE, 2017. 100580H (Proceedings of SPIE; Vol. 10058).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - In vivo fibre optic fluorescence-based sensing is the use of synthesised fluorophores which interrogate the local environment via variation in their fluorescence emission, addressed through an optic fibre. However, the emission intensity is influenced by intrinsic factors such as photobleaching, quantitative factors like concentration dependency and background signals from autofluorescence of tissue and the delivery optical fibre. Many of these problems can be addressed by using time-resolved spectroscopy which measures variations in the fluorescent lifetime. We present a versatile fibre-based time-resolved spectrograph based on a CMOS SPAD line sensor capable of acquiring time and spectral resolved fluorescent lifetime data in a single measurement exploiting the time-correlated single photon counting (TCSPC) technique. It is shown that these TCSPC histograms enable the differentiation between autofluorescence of tissue and synthesized fluorophores, as well as the removal of unwanted fibre background through post-processed time-gating. As a proof-of-principle application the pH- dependent changes in fluorescent lifetime of 5-carboxyuorescein (FAM) are measured.

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Ehrlich K, Kufcsák A, Krstajić N, Henderson RK, Thomson RR, Tanner MG. Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays. In Gannot I, editor, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII. Vol. 10058. SPIE. 2017. 100580H. (Proceedings of SPIE). Available from, DOI: 10.1117/12.2252090