Fibre-based spectral ratio endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence

Helen E. Parker; James M. Stone; Adam D. L. Marshall; Tushar R. Choudhary; Robert R. Thomson; Kevin Dhaliwal; Michael G. Tanner

doi:10.1364/BOE.10.001856

Fibre-based spectral ratio endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence

Helen E. Parker, James M. Stone, Adam D. L. Marshall, Tushar R. Choudhary, Robert R. Thomson, Kevin Dhaliwal, Michael G. Tanner

Research output: Contribution to journal › Article › peer-review

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Abstract

Fibre-based optical endomicroscopy (OEM) permits high resolution fluorescence microscopy in endoscopically accessible tissues. Fibred OEM has the potential to visualise pathologies targeted with fluorescent imaging probes and provide an in vivo in situ molecular pathology platform to augment disease understanding, diagnosis and stratification. Here we present an inexpensive widefield ratiometric fibred OEM system capable of enhancing the contrast between similar spectra of pathologically relevant fluorescent signals without the burden of complex spectral unmixing. As an exemplar, we demonstrate the potential of the platform to detect fluorescently labelled Gram-negative bacteria in the challenging environment of highly autofluorescent lung tissue in whole ex vivo human lungs.

Original language	English
Pages (from-to)	1856-1869
Number of pages	14
Journal	Biomedical Optics Express
Volume	10
Issue number	4
Early online date	15 Mar 2019
DOIs	https://doi.org/10.1364/BOE.10.001856
Publication status	Published - 1 Apr 2019

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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abstract = "Fibre-based optical endomicroscopy (OEM) permits high resolution fluorescence microscopy in endoscopically accessible tissues. Fibred OEM has the potential to visualise pathologies targeted with fluorescent imaging probes and provide an in vivo in situ molecular pathology platform to augment disease understanding, diagnosis and stratification. Here we present an inexpensive widefield ratiometric fibred OEM system capable of enhancing the contrast between similar spectra of pathologically relevant fluorescent signals without the burden of complex spectral unmixing. As an exemplar, we demonstrate the potential of the platform to detect fluorescently labelled Gram-negative bacteria in the challenging environment of highly autofluorescent lung tissue in whole ex vivo human lungs.",

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AU - Thomson, Robert R.

AU - Dhaliwal, Kevin

AU - Tanner, Michael G.

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Fibre-based spectral ratio endomicroscopy for contrast enhancement of bacterial imaging and pulmonary autofluorescence

Abstract

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