@inbook{a02b2d24f0e2495dab5edc9a3bd67ac6,
title = "Extraction of Microbial Cells from Environmental Samples for FISH Approaches",
abstract = "Fluorescent in situ hybridization (FISH) on environmental samples has become a standard technique to identify and enumerate microbial populations. However, visualization and quantification of cells in environmental samples with complex matrices is often challenging to impossible, and downstream protocols might also require the absence of organic and inorganic particles for analysis. Therefore, quite often microbial cells have to be detached and extracted from the sample matrix prior to use in FISH. Here, details are given for a routine protocol to extract intact microbial cells from environmental samples using density gradient centrifugation. This protocol is suitable and adaptable for a wide range of environmental samples.",
keywords = "Cell extraction, Environmental samples, FISH, Microbial cells, Microbial ecology",
author = "Jennifer Pratscher",
note = "Funding Information: JP was supported by a NERC Independent Research Fellowship (NE/L010771/2). Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
doi = "10.1007/978-1-0716-1115-9_19",
language = "English",
isbn = "9781071611142",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "291--299",
editor = "Azevedo, {Nuno F.} and Carina Almeida",
booktitle = "Fluorescence In-Situ Hybridization (FISH) for Microbial Cells",
address = "United States",
}